Immobilization of enzymes and proteins is a requirement for many industrial enzyme applications, as this facilitates enzyme recovery and reuse. Bearing in mind this necessity, the coupling of immobilization to the improvement of other enzyme features has been pursued by many researchers, and nowadays immobilization is recognized as a tool to improve not only stability, but also enzyme selectivi...

Identification, monitoring and quantification of biomolecules in the CNS is a field of growing interest for identifying biomarkers of neurological diseases. Implantable enzymatic microelectrode biosensors are a unique minimally invasive tool for real-time monitoring. Progress in microfabrication technologies has opened new possibilities in the preparation of complex biosensor architectures with...

Between 1930 and 1975 biochemical and structural analysis of enzymes led to a clear set of ideas that might form a basis for detailed understanding of enzyme action. Further development required energetic and thermodynamic analysis of enzymes in an aqueous medium, beyond the computational power then available. Structural enzymology advanced in other directions, but the fundamental questions of ...

Enzyme immobilization has become a key strategy to improve the stability and recycling of biocatalysts, resulting in greener more cost-efficient processes. The design immobilized catalysts is often focused only on strategy, binding chemistry between enzyme support, while less attention been paid physico-chemical properties material supports. Selecting best carrier for specific application may g...

We report here a novel bioelectrode based on self-assembled multilayers of polyphenol oxidase intercalated with cationic polyallylamine built up on a thiol-modified gold surface. We use an immobilization strategy previously described by Hodak J. et al. (Langmuir 1997, 13, 2708-2716) Quartz crystal microbalance with electroacustic impedance experiments were carried out to follow quantitatively t...

The biotechnological applications use stable and eco-friendly biocatalysts, with good operational properties, reusable easily separable. An efficient method of improving the stability enzymes as biocatalysts may be immobilization. immobilization research objectives are obtaining versatile insoluble enzyme preparations high enzymatic activity, capable catalyzing reactions their natural unnatural...

Bioelectrocatalysis using redox enzymes appears as a sustainable way for biosensing, electricity production, or biosynthesis of fine products. Despite advances in the knowledge parameters that drive efficiency enzymatic electrocatalysis, weak stability bioelectrodes prevents large scale development bioelectrocatalysis. In this review, starting from understanding protein instability, we will dis...