‘J’HE MOST COMMONLY USED METHODS for the estimation of uric acid are those colorimetric procedures utilizing the reduction of phosphotungstic acid (1-7). Since these procedures are not specific for uric acid, an enzymatic procedure utilizing uricase, which oxidizes uric acid to allantoin, has been employed by a number of investigators (8-12). The enzymatic method, dependent upon the absorption ...

A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, w...

a new method for the synthesis of water soluble polyaniline (pani) using hemin chloride (h.c) in the presence of hydrogen peroxide (h.p) is presented. hemin chloride is an effective catalyst for the oxidative polymerization of aniline in the presence of hydrogen peroxide at room temperature. the uv- vis absorption spectra of the product show a distinct absorption peak at 430 nm in ph 4.0 b...

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A simple enzymatic method for the determination of lactic acid with muscle lactic dehydrogenase is described. L ( +) Lactate in amounts from 0.04 M to 0.4 , M can be directly measured by following the formation of reduced diphosphopyridine nucleotide at 340 m . The effect of varying the concentrations of reactants in the test system was studied, and the optimal concentrations for stoichiometric...