Some characteristic enzymatic activities were determined in chick embryonic cartilage and compared with the analogous activities in bone and liver. Chondrocytes were isolated, broken by sonication, and subjected to subcellular fractionation to yield a nuclear pellet, the mitochondrial, lysosomal, and microsomal fractions, and the high speed supernatant solution. It was established that these fr...

The measurement of the plasma activities of glutamic-oxaloacetic and glutamic-pyruvic transaminases, aldolase, cholinesterase, and isocitric, lactic, and phosphogluconic dehydrogenases in random samples of blood was found to be of no value in the differential diagnosis of hepatitis, obstructive jaundice, hepatic cirrhosis, and neoplastic conditions involving the liver. Serial determinations of ...

AIM To investigate the role of erythrocyte free radical scavenging enzyme activities (FRSE), carbonic anhydrase (CA) activity and malondialdehyde (MDA) in infants with myelomeningocele (MM). MATERIAL AND METHODS We compared antioxidant enzyme activities and MDA level in 40 individuals (10 infants with MM, 10 healthy infants; and mothers of these two groups) with age-matched subjects. Erythroc...

A comparison was made of activities of 15 different enzymes in normal tissue samples of the inferior vena cava and aorta derived from the same subjects. Statistically significant differences between venous and arterial specimens were observed for 10 of these enzymes. Comparative studies on the intermediary metabolism of the vascular walls may disclose some of the factors responsible for the low...

Reliable and standardized methods are necessary to determine the expression of antioxidative enzymes and their role in maintaining health. In addition, the variability of the enzyme activities within the general population caused by age, gender, and life-style factors must be described. This study describes methodological conditions that are suitable for analyzing copper-zinc superoxide dismuta...

Results of clinical chemistry tests are often initially interpreted by relatively inexperienced junior medical staff, and therefore a major role of clinical chemists is to ensure that their laboratory tests are utilized and interpreted appropriately. The cases described below demonstrate the interaction of a diagnostic problem and clinical inexperience in an area where clinical chemists can, an...

this study was undertaken to estimate specific activities of rhodanese and 3-mercaptopyruvate sulfurtransferase (mst) in different tissues of turkey. enzyme activities were determined in tissue samples from freshly killed adult male turkeys by measurement of thiocyanate amounts produced by the action of enzymes on suitable substrates. activities of rhodanese and mst in examined tissues of turke...