Various solubilizers were tried during preformulation study. Propylene glycol and Transcutol were selected as solubilizers for further study. Poloxamer 407 gives gelation temperature in nasal temperature range 280 to 350 in 18% w/v concentration. Addition of bioadhesive polymer from 0.2% to 0.7% further lowered the gelation temperature from 340 to 290. Gelation temperatures obtained using two d...

A generalizable method for detecting protease activity via gelation is described. A recognition sequence is used to target the protease of interest while a second protease is used to remove the residual residues from the gelator scaffold. Using this approach, selective assays for both MMP-9 and PSA are demonstrated.

UNLABELLED The Thermodynamics of Enzyme-catalyzed Reactions Database (TECRDB) is a comprehensive collection of thermodynamic data on enzyme-catalyzed reactions. The data, which consist of apparent equilibrium constants and calorimetrically determined molar enthalpies of reaction, are the primary experimental results obtained from thermodynamic studies of biochemical reactions. The results from ...

Application of lipases (preferentially Candida antarctica Lipase B, CALB) for melt polycondensation aliphatic polyesters by transesterification activated dicarboxylic acids with diols allows to displace toxic metal and oxide catalysts. Immobilization the enzyme enhances activity temperature range use. The possibility use enzyme-catalyzed in is studied compared results polycondensations solution...

esterification of chitosan with adipic acid catalyzed by immobilized candida antarctica lipase b was carried out in this study. response surface methodology (rsm) based on a four-factor- five-level small central composite design (sccd) was employed to model and analyze the reaction. a total of 21 experiments representing different combinations of the four reaction parameters including ch...