Carbon film resistor electrodes have been evaluated as transducers for the development of multiple oxidase-based enzyme electrode biosensors. The resistor electrodes were first modified with Prussian Blue (PB) and then covered by a layer of covalently immobilized enzyme. Electrochemical impedance spectroscopy was used to characterize the interfacial behaviour of the Prussian Blue modified and e...

In the present study, glucose oxidase was produced in shake culture by the parent Aspergillus niger with corn steep liquor as substrate. Glucose oxidase produced was then purified by subjecting to 60-85% saturation of ammonium sulfate precipitation and further purified by ion exchange and gel filtration chromatography respectively. Desalted sample of glucose oxidase showed the activity and spec...

A B S T R A C T Human neutrophils stimulated with phorbol myristate acetate were able to destroy suspensions or monolayers of cultured human endothelial cells. Neutrophil-mediated cytotoxicity was related to phorbol myristate acetate concentration, time of incubation and neutrophil number. Cytolysis was prevented by the addition of catalase, while superoxide dismutase had no effect on cytotoxic...

Reaction of peroxides with 5-deazaflavin bound to glucose oxidase, lactate oxidase, or D-amino acid oxidase results in the formation of 5-deazaflavin 4a, 5-epoxide. The reaction of D-amino acid oxidase with m-chloroperoxybenzoate is an exception since the reagent reacts rapidly with the protein moiety to form m-chlorobenzoate which then binds noncovalently near the unmodified coenzyme. Epoxide ...

We report here that cultured human lymphoma cells in the absence of sonicated eosinophils are sensitive to killing by glucose oxldase (@3-Dglucose:oxygen..oxido reductase; EC 1.1.3.4) at concentrations as low as 0.025pg/mI,a levelthat canberapidlyattainedin s.c.tumor implantsin mice that receive a single nonlethal injection of enzyme. Multiple clono genie assays were used to measure the surviva...

Abstract Background Fungal glucose dehydrogenases (GDHs) are FAD-dependent enzymes belonging to the glucose-methanol-choline oxidoreductase superfamily. These classified in “Auxiliary Activity” family 3 (AA3) of Carbohydrate-Active enZymes database, and more specifically subfamily AA3_2, that also includes closely related flavoenzymes aryl-alcohol oxidase 1-oxidase. Based on sequence similarity...

Newly synthesized Polypropylene-g-Polyethylene glycol polymer was first time used for a compartment-less enzymatic fuel cell. Working electrodes based on Polypropylene-g-Polyethylene glycol were operated as unmediated and mediated system (with ferrocene and gold/cobalt oxide nanoparticles). Glucose oxidase and bilirubin oxidase was selected as anodic and cathodic enzyme, respectively. Glucose w...

We report the first demonstration of electrochemical detection for paper-based microfluidic devices. Photolithography was used to make microfluidic channels on filter paper, and screen-printing technology was used to fabricate electrodes on the paper-based microfluidic devices. Screen-printed electrodes on paper were characterized using cyclic voltammetry to demonstrate the basic electrochemica...

Previous work illustrated that glucose oxidase (GOx) could be deposited on conducting substrates using asymmetrical alternating current electrophoretic deposition (AC-EPD) to form thick enzyme layers suitable for the manufacturing of highly active biosensors. Here, we modeled the amperometric response of GOx layers to glucose as a function of the thickness of the enzyme layer. The model is base...

We present a rate-determination method for analyzing glucose. A glucose enzyme electrode serves as the sensor and is made by placing a gel-immobilized layer of glucose oxidase over the tip of a Clark-type O2 electrode. The electrode membrane is made of Teflon and is derivatized by etching with a suspension of colloidal sodium metal in organic solvent. The enzyme is coupled to the membrane surfa...