Objective: Isocorydine (ICD), an aporphine alkaloid, plays a role in anticancer activity that is still being evaluated. However, the exact function and mechanism of ICD endometrial carcinoma largely unknown. Methods: MTT, flow cytometry, western blot, immunofluorescence, RT-PCR ELISA were used this research. Results: In our study we showed inhibited HEC-1B cell proliferation at certain times co...

Prostate cancer stem-like cells (PCSCs) are being intensely investigated largely owing to their contributions towards prostate tumorigenesis, however, our understanding of PCSC biology, including their critical pathways, remains incompletely understood. While epidermal growth factor (EGF) is widely used in maintaining PCSC cells in vitro, the importance of EGF-dependent signaling and its downst...

OBJECTIVE Bone Morphogenetic Protein-4 (BMP-4) and Extracellular-Signal Regulated Kinases (ERK) play crucial roles in vascular diseases. Here, we demonstrate that BMP-4 not only signals through the classical Smad cascade but also activates ERK phosphorylation as an alternative pathway in human umbilical vein endothelial cells (HUVEC) and that Smad and ERK pathways communicate through signal cro...

Inactivating mutations in the adenomatous polyposis coli gene (APC), and activating mutations in RAS, occur in a majority of colorectal carcinomas. However, the relationship between these changes and tumorigenesis is poorly understood. RAS-induced activation of the ERK pathway was reduced by overexpressing APC in DLD-1 colorectal cancer cells. ERK activity was increased by Cre-virus-induced Apc...

Two major intracellular signals that regulate neuronal function are calcium and cAMP. In many cases, the actions of these two second messengers involve long term changes in gene expression. One well studied target of both calcium and cAMP signaling is the transcription factor cAMP-responsive element-binding protein (CREB). Multiple signaling pathways have been shown to contribute to the regulat...

To figure out the molecular mechanism of aloperine (ALO) on receptor activator nuclear factor (NF)-kappa-B (κb) ligand (RANKL)-caused osteoclast differentiation. The histopathological analysis and tartrate-resistant acid phosphatase (TRAP) staining assays were applied to check extent bone loss femur. Then, protein expressions nitric oxide synthase 2, glutathione reductase, erythroid 2-related h...

urokinase plasminogen activator receptor (upar) and its ligands play a major role in many tumors by mediating extracellular matrix degradation and signaling cascades leading to tumor growth, invasion and metastasis. recently we introduced upar decapeptide antagonist with cytotoxic effect on mda-mb-231 cell line. in this study we assessed the alteration in upar downstream signaling following tre...

Background The hypothesis that the LDA (low-dose-aspirin) could improve ovarian and uterine perfusion induced clinicians to administer it in women undergoing IVF. Unfortunately, no studies have shown significant differences in terms of pregnancy rate among patients treated or untreated with LDA. The absence of proven clinical benefits of LDA supplementation, led us to wonder whether aspirin, ad...

OBJECTIVES Naringin has been reported to regulate bone metabolism. However, its effect on osteogenesis remains unclear. The aim was to investigate the effect of naringin on osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) through the activation of the ERK signaling pathway in osteogenic differentiation. MATERIALS AND METHODS Annexin V-FITC assay and MTT assay we...