Inseticidas organofosforados, como o clorpirifos, exercem sua toxicidade pela inibição de enzimas esterases, tais a acetilcolinesterase (AChE) e carboxilesterase (CbE) em insetos outros animais. Por isso, avaliação da atividade destas é amplamente utilizada biomarcadora exposição animais estes tipos compostos, estudos monitoramento ambiental. No presente estudo, compreensão desses efeitos foi t...

Under axenic growth all 14 syngens of Paramecium aurelia have 4 types of esterases. The three major types (A, B and cathodal C) vary independently in electrophoretic mobility among the syngens. Using these three esterases, stocks can be keyed to a syngen, except for the groupings 1-3-5 and 7-13. Using 5 other enzymes only syngens 1 and 5 cannot be distinguished. Most syngens differ from each ot...

sideration in the literature [13]. As a result, the findings obtained in this investigation may be applicable to the study of PMA in other cell systems. According to Augustinsson [S], each esterase type exists in multiple forms and each animal species has its own typical spectrum of esterases in plasma and tissues [14]. In the presence of marked variations in location, form and activity, one mi...

Two mutations have been found in five closely related insect esterases (from four higher Diptera and a hymenopteran) which each confer organophosphate (OP) hydrolase activity on the enzyme and OP resistance on the insect. One mutation converts a Glycine to an Aspartate, and the other converts a Tryptophan to a Leucine in the enzymes' active site. One of the dipteran enzymes with the Leucine mut...

The aim of the present work was to study the deltamethrin susceptibility of eggs from Triatoma infestans populations and the contribution of pyrethroid esterases to deltamethrin degradation. Insects were collected from sylvatic areas, including Veinte de Octubre and Kirus-Mayu (Bolivia) and from domiciliary areas, including El Palmar (Bolivia) and La Pista (Argentina). Deltamethrin susceptibili...

The major insecticide resistance mechanism in the brown planthopper Nilaparvata lugens involves overproduction of esterases. Esterases purified from a resistant strain appeared as a ladder of bands on isoelectric focussing (IEF) gels from pI 4.7 to 5.0. Two-dimensional electrophoresis showed that isozymes ranged in size from 66 to 68 kDa with those of lower pI being apparently smaller. All isoz...

Reactions catalysed by sustainably produced enzymes can contribute to the bioeconomy supporting several industries. Low-value compounds be transformed into added-value products or high-resolution chemicals could prepared in reactions biocatalyst esterase enzymes. These synthesised purposely isolated genetically modified strains of microorganisms. Enzymes belonging hydrolase family catalyse form...

We have investigated the basis of adhesion of uredospores of the obligately parasitic rust fungus Uromyces viciae-fabae to leaves of its broad bean host. Upon contact with an aqueous environment, spores form a structure that we have termed an adhesion pad. The adhesion pad is formed by both living and autoclaved spores, but only adhesion pads formed by living spores adhered to the cuticle of le...

Lipolytic enzymes catalyze the hydrolysis of ester bonds in the presence of water. In media with low water content or in organic solvents, they can catalyze synthetic reactions such as esterification and transesterification. Lipases and esterases, in particular those from extremophilic origin, are robust enzymes, functional under the harsh conditions of industrial processes owing to their inher...