نتایج جستجو برای: ests
تعداد نتایج: 2273 فیلتر نتایج به سال:
Using PCR-select subtractive cDNA hybridization technique, 41 in vitro expressed sequence tags (ESTs) were isolated from a modified live vaccine strain (AQUAVAC-ESC, formerly RE-33) vs a virulent parent strain (EILO) of Edwardsiella ictaluri. Transcriptional levels of the 41 in vitro ESTs in the vaccine strain and the virulent strain were then evaluated by quantitative PCR (qPCR). The qPCR resu...
Abstract The current natural extract modalities for colorectal cancer are limited. This research seeks to assess the process of extracting plant’s bioactive constituents from Cassia angustifolia and show anticancer role played by aqueous at 0°C identifying genes that alter in expression after treatment colon cells. components were revealed using gas chromatography-mass spectrometry analysis. ca...
BACKGROUND There is little knowledge around the world about training and certification for general thoracic surgeons, and the relationship between thoracic surgery and cardiac surgery. Examination of the membership of European Association for Cardio-Thoracic Surgery (EACTS) and European Society of Thoracic Surgeons (ESTS) can clarify the training, practice and academic activity of European thor...
Single pass sequences of mRNA, called ESTs, have been determined extensively. They have been accumulated in the dbEST database in GenBank. The number of ESTs in dbEST has become more than eight million in August 2002. By clustering and assembling ESTs, we can conduct the following analyses. First, we can obtain complete ORF sequences based on ESTs that are fragment sequences of mRNA and do not ...
Analysis of transcriptomes is of great importance in genomic studies. Asian seabass is an important fish species. A number of genomic tools in it were developed, while large expressed sequence tag (EST) data are lacking. We sequenced ESTs from nine normalized cDNA libraries and obtained 11 431 high-quality ESTs. We retrieved 8524 ESTs from dbEST database and analyzed all 19 975 ESTs using bioin...
Large-scale single-pass sequencing of cDNAs from different plants has provided an extensive reservoir for the cloning of genes, the evaluation of tissue-specific gene expression, markers for map-based cloning, and the annotation of genomic sequences. Although as of January 2000 GenBank contained over 220,000 entries of expressed sequence tags (ESTs) from plants, most publicly available plant ES...
Prickly lettuce (Lactuca serriola L.) is a problematic weed of Pacific Northwest and recently developed resistance to the auxinic herbicide 2,4-D. There are no publically available simple sequence repeat (SSR) markers to tag 2,4-D resistance genes in L. serriola. Therefore, a study was conducted to develop SSR markers from expressed sequence tags (ESTs) of 5 Lactuca species. A total of 15,970 S...
We report 80,388 ESTs from 23 Atlantic salmon (Salmo salar) cDNA libraries (61,819 ESTs), 6 rainbow trout (Oncorhynchus mykiss) cDNA libraries (14,544 ESTs), 2 chinook salmon (Oncorhynchus tshawytscha) cDNA libraries (1317 ESTs), 2 sockeye salmon (Oncorhynchus nerka) cDNA libraries (1243 ESTs), and 2 lake whitefish (Coregonus clupeaformis) cDNA libraries (1465 ESTs). The majority of these are 3...
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