Ethylenediurea (EDU), N[2-(2-oxo-l-imidazolidinyl) ethyl]-N'-phenylurea is known to prevent ozone (03) damage to leaf tissues. However, the mechanisms of protection are unclear. We tested the hypothesis that EDU protects against 03 damage by scavenging hydroxyl free radicals (OH). An in vitro study involving the use of high-performance liquid chromatography equipped with an electrochemical dete...

A novel technique using the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) into replicating DNA is described for the analysis of replicating banding patterns of human metaphase chromosomes. Human lymphocytes were synchronized with excess thymidine and treated with EdU during the late S phase of the cell cycle. The incorporated EdU was then detected in metaphase chromosomes using Alexa Fluor® ...

We report procedures to allow incorporation and detection of 5-ethynyl-2'-deoxyuridine (EdU) in fission yeast, a thymidine analogue which has some technical advantages over use of bromodeoxyuridine. Low concentrations of EdU (1 µM) are sufficient to allow detection of incorporation in cells expressing thymidine kinase and human equilibrative nucleoside transporter 1 (hENT1). However EdU is toxi...

BACKGROUND Neointimal formation plays an important role in the pathogenesis of coronary restenosis after percutaneous coronary intervention (PCI), especially in patients with diabetes mellitus. Recently, some studies have shown that 5-ethynyl-2'-deoxyuridine (EdU) incorporation can serve as a novel alternative to the 5-bromo-2'-deoxyuridine (BrdU) antibody detection method for detection of DNA ...

1 Computer and Information Sciences Department, 101A Smith Hall, University of Delaware, Newark, DE 19716-2586, U.S.A., case~cis .udel .edu. 2 Computer Science, Sacred Heart University, 5151 Park Avenue, Fairfield, CT 06432-1000, U.S.A., kinberCshu, sacredheart , edu. 3 School of Computer Science and Engineering, The University of New South Wales, Sydney, NSW, 2052, Australia, arun 9 unsw. edu....

partially supported by NSF grant CCR and NSF grant CCR Internet kim cs williams edu yInternet luca src dec com zInternet castagna dmi ens fr xJonathan Eifrig Scott Smith Valery Trifonov Contact Scott Smith Research partially supported by NSF grant CCR and AFOSR grant F Internet scott cs jhu edu partially supported by NSF grants CCR and CCR Internet leavens cs iastate edu kInternet benjamin pier...

Department of Physics University of Washington P O Box Seattle WA USA bulgac phys washington edu http www phys washington edu bulgac Giu DO DANG Laboratoire de Physique Th eorique et Hautes Energies Universit e de Paris Sud B at Orsay FRANCE Giu Dodang th u psud fr Dimitri KUSNEZOV Center for Theoretical Physics Sloane Physics Laboratory Yale University New Haven CT USA dimitri nst physics yale...

BACKGROUND The kidney is a specialized low-regenerative organ with several different types of cellular lineages. The BrdU label-retaining cell (LRCs) approach has been used as part of a strategy to identify tissue-specific stem cells in the kidney; however, because the complementary base pairing in double-stranded DNA blocks the access of the anti-BrdU antibody to BrdU subunits, the stem cell m...