In this study, two new terpyridine-based EuIII complexes were synthesized, the structures of which were optimized for luminescence resonance energy-transfer (LRET) experiments. The complexes showed high quantum yields (32 %); a single long lifetime (1.25 ms), which was not influenced by coupling to protein; very high stability in the presence of chelators such as ethylenediamine-N,N,N',N'-tetra...

Structural proteins of the poultry pathogen, infectious bursal disease virus (IBDV), were expressed in the baculovirus/insect cell expression system. To date, several reports have indicated that animal virus structural proteins are expressed only at low yield in this system. In this article, several factors were examined to enhance yield. These include medium, dissolved oxygen level, and the ad...

Materials and Methods: Twenty-six children with serous otitis media, ages ranging between 4-14 years were prospectively included into the study. A diagnosis of OME was made on the basis of the patient’s history and examination, otoscopy, tympanogram and pure tone audiometry. The MEEs were collected via a sterile, disposable aspirator (Juhn Tym-Tap®, Xomed Inc., Jacksonville, FL 32216, USA) afte...

Removal of copper and nickel by the addition of the biodegradable chelating agent, chitosan and ethylenediamine tetraacetic acid (EDTA), was investigated, alongside the reaction of a reference compound sodium citrate for comparison. The artificial-contaminated soils were used in this study. The experiments showed that the extraction ability for copper and nickel from the contaminated soil decre...

Root diffusate from soybean plants caused greater hatching of Heterodera glycines eggs during vegetative growth of the host, but the activity declined with plant senescence. Chelation of the root diffusate with ethylenediamine tetraacetic acid (EDTA) significantly increased hatching activity for H. glycines eggs. Diffusate from leafless plants caused little hatching, whereas treatment of intact...

HE RENEWED INTEREST in magnesium alterations in human disease states has originated a search for simpler and more accurate analytic technics for the determination of magnesium in body fluids. Several recent methods are based upon the chelating reaction of ethylenediamine tetraacetic acid (EDTA). A procedure for the determination of magnesium in erythrocytes (1) involving spectrophotometric titr...

BIOLOGICAL BEHAVIOR OF K58MnO4: ITS ACCUMULATION IN NORMAL TISSUES AND PATHOLOGICAL BRAIN J. E. Roth,A. H. Soloway, P. Bendaand W. H. Sweet COMPUTER-FOCUSING USING AN APPROPRIATE GAUSSIAN FUNCTION Teruo Nagai, Nobuo Fukuda and T. A. linuma DIAGNOSIS OF SPONDYLITIS BY EXTERNAL 85Sr COUNTING: A COMPARISON OF ANALYTIC PROCEDURES Lars Lindberg and Melvin S. Schwartz PANCREATIC CONCENTRATION OF 1251...

The synthesis and characterization of a Keggin-type mono-rhenium(V)substituted polyoxotungstate are described. The dimethylammonium salt [Me2NH2]4[PW11ReO40] was obtained as analytically pure homogeneous black-purple crystals by reacting mono-lacunary Keggin polyoxotungstate with [ReCl6] in water, followed by crystallization from acetone at ca. 5 °C. Single-crystal X-ray structural analysis of ...

Sexual dimorphism of mouse complement component 5 (C5) was detected by isoelectric focusing of desialated ethylenediamine tetraacetic acid (EDTA)-plasma on agarose gel, followed by immunofixation with anti-mouse C5. Male plasma displayed two C5 bands, basic and acidic, while female plasma had only a basic C5 band. In all mouse strains tested except C5-deficient strains, the identical patterns o...

Purified human pancreatic alpha-amylase (alpha-1,4-glucan 4-glucano-hydrolase, EC 3.2.1.1) was found to be stable over a wide range of pH values (5.0 to 10.5) with an optimal pH for the enzymatic activity of 7.0. The Michaelis constant of the enzyme at optimal pH and assay conditions was found to be 2.51 mg per ml for soluble starch. Halide ions were required for the activity of the enzyme wher...