Most functions attributed to Tityus serrulatus venom (TsV) are related to active molecules on ion-channels; however, here we describe a new pentapeptide that was discovered through enzymatic assay selection using EP24.15. The primary structure analysis revealed the sequence KEXXG (X means Ile or Leu), similar to the sequence present in the β-KTX propeptide described from the venom of Tityus spp...

The present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino ...

Toxicity of three mosquito oviposition repellents, N,N-diethyl-3-methylbenzamide (AI3-22542 or deet), AI3-35765, and AI3-37220 to 6 aquatic nontarget invertebrates, was evaluated in the laboratory. The 24-h LC50 values for Cypricercus sp. (Ostracoda), Moina sp. (Cladocera), Eucyclops agilis Koch (Copepoda), Strelkovimermis spiculatus Poinar & Camino (Nematoda), Þrstand fourth-instar Toxorhynchi...

Cyclopid copepods from samples of fauna associated with aquatic macrophytes and plancton obtained in lotic and lentic environments were obtained from the upper Paraná River floodplain (in the states of Paraná and Mato Grosso do Sul, Brazil). Macrophytes were collected in homogeneous stands and washed. Plankton samples, taken from the water column surface and bottom, were obtained using a motor ...

Objective To identify areas that present a higher risk of exposure to accidents with scorpions in Brazil. Methods We used techniques spatial prioritisation determine the most vulnerable localities envenomation by four scorpion species. Our integrated ecological niche models health investment, antivenin availability, access care facilities and metrics human impact data. Results The indicated thr...

To date, several families of peptide toxins specifically interacting with ion channels in scorpion venom have been described. One of these families comprise peptide toxins (called KTxs), known to modulate potassium channels. Thus far, 202 KTxs have been reported, belonging to several subfamilies of KTxs (called α, β, γ, κ, δ, and λ-KTxs). Here we report on a previously described orphan toxin fr...