Aneuploidy has been well-documented in blastocyst embryos, but prior studies have been limited in scale and/or lack mechanistic data. We previously reported preclinical validation of microarray 24-chromosome preimplantation genetic screening in a 24-h protocol. The method diagnoses chromosome copy number, structural chromosome aberrations, parental source of aneuploidy and distinguishes certain...

Influenza-associated excess mortality is widely used to assess the severity of influenza epidemics. In Germany, however, it is not yet established as a routine component of influenza surveillance. We therefore applied a simple method based on the annual distribution of monthly relative mortality (relative mortality distribution method, RMDM) to a time-series of German monthly all-cause mortalit...

In recent years, numerous groups of investigators have reported primary aldosteronism (PAL) to be much more common than previously thought.1–4 This has followed recognition of the aldosterone/renin ratio (ARR) as an improved, more sensitive method of screening for PAL and the application of ARR testing to a wider population, to include normokalemic, as well as hypokalemic, hypertensive subjects...

Molecular cytogenetic methods were applied to investigate the effect of the occupational exposure to low concentrations of benzene and petroleum fuels on genomic stability. Twelve male gasoline station attendants (average benzene exposure of 0.32 mg/m3 as 8h TWA) and 12 age- and smoking-matched unexposed controls were selected for the study. The incidence of hyperploidy and polyploidy in periph...

Myelodysplastic syndromes (MDSs) are clonal hematologic disorders that frequently represent an intermediate disease stage before progression to acute myeloid leukemia (AML). As such, study of MDS/AML can provide insight into the mechanisms of neoplastic evolution. In 184 patients with MDS and AML, DNA methylation microarray and high-density single nucleotide polymorphism array (SNP-A) karyotypi...

Myelodysplastic syndromes (MDSs) are clonal hematologic disorders that frequently represent an intermediate disease stage before progression to acute myeloid leukemia (AML). As such, study of MDS/AML can provide insight into the mechanisms of neoplastic evolution. In 184 patients with MDS and AML, DNA methylation microarray and high-density single nucleotide polymorphism array (SNP-A) karyotypi...

A man with cytopenias, dysplasia, excess blasts, P53 and RUNX1 mutations, and ring chromosome 7 recovered after stopping lenalidomide.

The effect of prefixation on the diameter of chromosome fibers isolated by the Langmuir trough-critical point method has been investigated in several species of plants and animals. In barley, fibers isolated from endosperm without prefixation have an average diameter of between 240 and 250 A, and are similar in dimensions and structure to the chromosome fibers isolated from animals by this meth...

The effect of prefixation on the diameter of chromosome fibers isolated by the Langmuir trough-critical point method has been investigated in several species of plants and animals. In barley, fibers isolated from endosperm without prefixation have an average diameter of between 240 and 250 A, and are similar in dimensions and structure to the chromosome fibers isolated from animals by this meth...

Background: Several epidemiological studies conducted on underground miner’s show that exposure to elevated levels of radioactive radon gas is expected to increase the risk of lung cancer. Relative risk of lung cancer is almost linear with radon exposure. Elevated concentrations of radon are not only reported within mines but also for closed indoor environment of general public houses....