نتایج جستجو برای: exoenzyme s

تعداد نتایج: 711274  

Journal: :Infection and immunity 1989
J Coburn S T Dillon B H Iglewski D M Gill

Exoenzyme S, which had been thought to be unselective, catalyzes the ADP-ribosylation of only a subset of cellular proteins. The intermediate filament protein vimentin is one of the more abundant substrates. Disassembled vimentin, and proteolytic fragments of vimentin that cannot form filaments, is more readily ADP-ribosylated than is filamentous vimentin.

Journal: :The Journal of Cell Biology 1993
T Tominaga K Sugie M Hirata N Morii J Fukata A Uchida H Imura S Narumiya

Botulinum C3 exoenzyme specifically ADP-ribosylates a group of ras-related small molecular weight GTP-binding proteins, rho, and inhibits their biological activity. Using this enzyme, we examined the function of rho in PMA-induced activation of lymphocyte function-associated antigen-1 (LFA-1) in a B lymphoblastoid cell line, JY. Northern blot analysis revealed that among the three rho genes, rh...

Journal: :FEMS microbiology letters 2004
Charlotta Sundin Bengt Hallberg Ake Forsberg

Pseudomonas aeruginosa utilises a type III secretion system (TTSS) to introduce exoenzyme S and exoenzyme T into host cells to subvert host cell signalling and thereby promote infection. In this study, we have employed the heterologous TTSS of Yersinia to deliver different mutants of ExoT into HeLa cells. Wild-type ExoT and ExoT variants expressing either GAP (GTPase activating protein) or ADP-...

Journal: :Biotechnology journal 2010
Stefan Schuster Jan-Ulrich Kreft Naama Brenner Frank Wessely Günter Theissen Eytan Ruppin Anja Schroeter

The engineering of microorganisms to produce a variety of extracellular enzymes (exoenzymes), for example for producing renewable fuels and in biodegradation of xenobiotics, has recently attracted increasing interest. Productivity is often reduced by "cheater" mutants, which are deficient in exoenzyme production and benefit from the product provided by the "cooperating" cells. We present a game...

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