Fibroblasts deficient in beta-glucuronidase acquired high levels of this enzyme when they were co-cultured with concanavalin A-stimulated lymphocytes. Acquired enzyme activity, determined using a single-cell cytochemical assay, was directly proportional to the number of lymphocytes added and persisted for several days in fibroblasts maintained at high density. Lymphocytes did not secret signifi...

BACKGROUND Conventional corn dry-grind ethanol production process requires exogenous alpha and glucoamylases enzymes to breakdown starch into glucose, which is fermented to ethanol by yeast. This study evaluates the potential use of new genetically engineered corn and yeast, which can eliminate or minimize the use of these external enzymes, improve the economics and process efficiencies, and si...

Catfish (Clarias gariepinus) is a freshwater fish that widely cultivated in Indonesia. Production cost of farming activities spend 60-70% on the use feed. Therefore, feeding must be effective and efficient so catfish can make good it for its growth. The addition an exogenous enzyme form papain increase efficiency feed utilization. purpose this study was to determine effect growth survival sangk...

Enzymes are appealing diagnostic targets because of their centrality in human health and disease. Continuous efforts spanning several decades have yielded methods for magnetically detecting the interactions enzymes with exogenous molecular substrates. Nevertheless, measuring enzymatic activity vivo remains challenging due to background noise, insufficient selectivity, overlapping functions. Mag...

Endothelial nitric oxide synthase (eNOS) is active only as a homodimer. Recent data has demonstrated that exogenous NO can act as an inhibitor of eNOS activity both in intact animals and vascular endothelial cells. However, the exact mechanism by which NO exerts its inhibitory action is unclear. Our initial experiments in bovine aortic endothelial cells indicated that exogenous NO decreased NOS...

Background: Ornithine (Orn) plays an essential role in the metabolism of plant cells through incorporation in polyamines biosynthesis, the urea cycle and nitrogen metabolism. Physiological response of the plant cells to its two enantiomers have not been widely investigated yet.Objectives: This study aimed to evaluate effect of ornithine enantiomers on exp...

the present study was carried out to investigate the effect of different levels of triticale with/ without enzyme cocktail (xylanase and β-glucanase) on performance of broiler chicks. five hundred day old male broiler chicks (ross 308), assigned to a factorial arrangement (5×2) with a completely randomized design with 5 replicates of 10 chicks each. the factors included 5 levels of triticale (0...

The effect of self-phosphorylation on the protein-tyrosine kinase activity of the epidermal growth factor receptor has been investigated using immunoaffinity-purified protein. Enzyme was first incubated for various times with excess ATP to phosphorylate it to differing extents; the ability of the enzyme to phosphorylate exogenous peptide substrates was then measured as a function of its self-ph...

A protein kinase activity has been found associated with purified Sendai virions. Most of the virion proteins and exogenous protamine served as substrates for this enzyme.

Twenty-eight day feeding trial was conducted to evaluate the performance of broiler finisher fed soybean hull based diets supplemented with “Safzyme®”, an exogenous cellulolytic enzyme. Soybean also referred as soyhull, millrun or flakes are by-products milling industry. Five were formulated such that contained meal at 0%, 10% and 20% dietary levels (without enzyme supplementation) 10%, (with 1...