نتایج جستجو برای: expression cassette

تعداد نتایج: 878293  

2014
MASAMI WATANABE MASAKIYO SAKAGUCHI RIE KINOSHITA HARUKI KAKU YUICHI ARIYOSHI HIDEO UEKI RYUTA TANIMOTO SHIN EBARA KAZUHIKO OCHIAI JUNICHIRO FUTAMI SHUN-AI LI PENG HUANG YASUTOMO NASU NAM-HO HUH HIROMI KUMON

Gene expression systems with various promoters, including the cytomegalovirus (CMV) promoter, have been developed to increase the gene expression in a variety of normal and cancer cells. In particular, in the clinical trials of cancer gene therapy, a more efficient and robust gene expression system is required to achieve sufficient therapeutic outcomes. By inserting the triple translational enh...

Hasan Marashi Maziar Habibi-Pirkoohi, Mohammadreza Nassiri, Nasrin Moshtaghi, Saeid Malekzadeh-Shafaroudi Saeid Zibaee

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

Journal: :Arteriosclerosis, thrombosis, and vascular biology 2015
Kai Yin Yong You Vicki Swier Lin Tang Mohamed M Radwan Amit N Pandya Devendra K Agrawal

OBJECTIVE Prevalence of vitamin D (VD) deficiency and its association with the risk of cardiovascular disease prompted us to evaluate the effect of VD status on lipid metabolism and atherosclerosis in hypercholesterolemic microswine. APPROACH AND RESULTS Yucatan microswine were fed with VD-deficient (0 IU/d), VD-sufficient (1000 IU/d), or VD-supplemented (3000 IU/d) high-cholesterol diet for ...

2016
Sally Partridge Béatrice Berçot Érica L. Fonseca Ana Carolina Paulo Vicente

The gene cassettes found in class 1 integrons are generally promoterless units composed by an open reading frame (ORF), a short 5’ untranslated region (UTR) and a 3’ recombination site ( ). Fused gene cassettes are generated attC by partial or total loss of the from the first cassette in an array, creating, in attC some cases, a fusion with the ORF from the next cassette. These structures are r...

Journal: :Current opinion in plant biology 1999
P N Benfey

Recently, it has been shown that the same sets of genes act in both root and shoot to regulate cell fate and patterning. One gene cassette regulates epidermal cell fate, another cassette regulates ground tissue derived cell fate and organization. Ectopic expression and laser ablation have been used to probe the mechanisms by which these genes perform their tissue and organ-specific functions.

Journal: :PLoS ONE 2007
Hoorig Nassanian Ana M. Sanchez Alice Lo Kenneth A. Bradley Benhur Lee

BACKGROUND RNA interference (RNAi), mediated by small interfering RNA (siRNA), is an effective method used to silence gene expression at the post-transcriptional level. Upon introduction into target cells, siRNAs incorporate into the RNA-induced silencing complex (RISC). The antisense strand of the siRNA duplex then "guides" the RISC to the homologous mRNA, leading to target degradation and gen...

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