In this work the Computational Fluid Dynamics simulation of a stirred bioreactor purposely designed for the production of hydrogen from organic wastes fermentation is presented. The bioreactor is a vortex-ingesting, dual impeller, mechanically-agitated vessel equipped with a central draft tube. Due to the geometrical and physical complexity, the selected reactor offers a very challenging benchm...

This paper presents a miniaturized bioreactor (bioreactor-on-a-chip) applied to baker’s yeast fermentation. The bioreactor-on-a-chip is fabricated using a silicon and glass wafers applying micromachining technology (wet-etching techniques) in order to create microchannels, mixerchannels, microvalves. The miniaturization and integration allows smaller volumes to be used, which can be often rathe...

We used the software Optferm to model and optimize the bioprocess of escherichia coli fermentation, simulating the conditions of a fed-batch bioreactor. The results, obtained by employing different AI approaches, provided useful orientation for nutrient feed strategies for increased productivity. In conclusion, we believe in silico tools can perform a rapid and valid optimization of bioprocesse...

Isopropanol-butanol fermentation carried out by C. beijerinckii can be improved immobilizing cells in a fixed bed bioreactor. In this study, bioreactor was developed using polyurethane foams as solid support for biofilm growth. The dilution rate increased over time to follow development while avoiding solvent accumulation within the A phenomenological model designed and successfully described b...

Variation in the hydrogen production rate was consistent with the succession of dominant bacteria during the batch fermentation process. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes and quantitative analysis of the hydA genes at both the DNA and mRNA levels confirmed that Clostridium perfringens was the most dominant hydrogen producer in the bioreactor.

Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus enabling for lactose metabolization. Data on yeast fermentation and growth on cheese whey permeate from a Portuguese dairy industry is presented. For...

One of the major steps toward the industrialization of the microbial product(s) is to optimize the cultivation conditions at the large-scale bioreactor and successfully control the microbial behavior within a large scale production environment. Statistical Design of Experiment was proven to optimize a vast number of microbial processes to achieve robustness and explore possible interactions...

The feasibility of second-generation (2G) processes still highly depends on improvements in the hydrolysis and fermentation steps, ensuring whole use biomass sugars an integrated approach. main bottlenecks are differences between optimal conditions for both limitations high solid load (HSL) hydrolysis, hexoses pentose co-fermentation. To overcome these problems, present work explored a feeding ...

Cassava constitutes an abundant substrate in tropical regions. The production of butanol in ABE fermentation by Clostridium beijerinckii BA101 using cassava flour (CF) was scaled-up to bioreactor level (5 L). Optimized fermentation conditions were applied; that is, 40℃, 60 g/l CF, and enzymatic pretreatment of the substrate. The batch fermentation profile presented an acidogenic phase for the f...

This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bi...