Fibrinogen-binding platelet proteins have been examined by crossed-immunoelectrophoresis of solubilized. washed platelets followed by the incubation of the immunoplates with 1251-fibrinogen and exposure to x-ray films. Incubation with 0.1 mg/mI of ‘2 l-fibrinogen revealed the binding of fibrinogen to the immunoprecipitates representing the glycoprotein lIb-Illa complex. factor Xllla chain, a gr...

BACKGROUND Fibrinogen concentrate may reduce blood loss after trauma. However, its effect on endogenous fibrinogen synthesis is unknown. The authors investigated the effect of exogenous human fibrinogen on endogenous fibrinogen metabolism in a 24-h porcine trauma model. METHODS Coagulopathy was induced in 20 German Landrace pigs by hemodilution and blunt liver injury. Animals were randomized ...

A 17 yr old female with a congenital bleeding disorder was found to suffer from dysfibrinogenemia. Whole blood and plasma coagulation times were delayed and thrombelastograms were grossly abnormal. Clottability of plasma fibrinogen by addition of thrombin was not demonstrated during the 30 min test period. Fibrinogen was revealed by turbidometric and immunologic techniques. Other coagulation fa...

Previous analysis of fibrinogen binding to human aspirin-treated gel-filtered platelets yielded upwardly concave Scatchard plots. To ascertain whether this was due to the presence of independent heterogeneous receptor populations binding fibrinogen with different affinities, the dissociation of purified 125I-fibrinogen from ADP-treated gel-filtered platelets was evaluated as a function of recep...

The Aα, Bβ and γ polypeptide chains of fibrinogen are encoded by a three gene cluster on human chromosome four. The fibrinogen genes (FGB-FGA-FGG) are expressed almost exclusively in hepatocytes where their output is coordinated to ensure a sufficient mRNA pool for each chain and maintain an abundant plasma fibrinogen protein level. Fibrinogen gene expression is controlled by the activity of pr...

Fibrinogen binding to receptors on stimulated platelets is a prerequisite for platelet aggregation. In order to identify the platelet fibrinogen receptor, we modified fibrinogen with the photoreactive, heterobifunctional cross-linking reagent methyl 4-azidobenzoimidate (MABI). MABI-fibrinogen was fully clottable and able to support platelet aggregation. To photoaffinity label the fibrinogen rec...

Fibrinogen production. measured by the rate of incorporation of 75Se-selenomethionine into newly synthesized circulating fibrinogen. was determined in rabbits administered urokinase and ancrod to generate degradation products of fibrinogen or fibrin in vivo. Fibrinogen degradation products appeared in elevated titers in metabolically normal animals after receiving infusions of urokinase. Fibrin...