Lung fibrosis is considered a severe manifestation of microscopic polyangiitis (MPA). Antimyeloperoxidase (anti-MPO) antibodies in MPA patients' sera can activate MPO and lead to the production of reactive oxygen species (ROS). While high levels of ROS are cytotoxic, low levels can induce fibroblast proliferation. Therefore, we hypothesised that the oxidative stress induced by anti-MPO antibodi...

AIMS Fibroblasts, which play an important role in cardiac function/dysfunction, including arrhythmogenesis, have voltage-dependent (Kv) currents of unknown importance. Here, we assessed the differential expression of Kv currents between atrial and ventricular fibroblasts from control dogs and dogs with an atrial arrhythmogenic substrate caused by congestive heart failure (CHF). METHODS AND RE...

Several recent studies have shown that proteins of the cadherin-catenin complex are not only involved in cell-cell adhesion but also in the proliferation and differentiation processes. For the first time, we investigated the effect of the quantity of cytoplasmic beta-catenin on dermal fibroblast proliferation by overexpressing human beta-catenin in human dermal fibroblasts. Our results show tha...

Keloids are hypertrophic and proliferating pathological scars extending beyond the initial lesion without any tendency to regression. Usually, keloids consideredand treated as a single entity but clinical observations suggest heterogeneity in keloid morphologies with distinction of extensive nodular entities. Within keloid, could also be detected between superficial deep dermis or center periph...

Background: Cancer refers to the abnormal growth of cells and is still the most common cause of morbidity in world. The purpose of this study was to determine cytotoxicity effect of high molecular weight (HMWC) and low molecular weight of chitosan (LMWC) on three cancerous cell lines MCF-7, HeLa and Saos-2 with different histological origin. Methods: The anticancer property of two types of chi...

Mast cells play a potentially important role in fibroproliferative diseases, releasing mediators including tryptase that are capable of stimulating fibroblast proliferation and procollagen synthesis. The mechanism by which tryptase stimulates fibroblast proliferation is unclear, although recent studies suggest it can activate protease-activated receptor (PAR)-2. We therefore investigated the ro...

scleromyxedema is a rare, chronic, progressive, fibromucinous disorder of unknown etiology, characterized by lichenoid waxy papules and firm induration of skin of trunk, face, forearm and hands; fibroblast proliferation and mucin deposition in the upper dermis. cutaneous involvement is characteristic but there are several associated systemic manifestations: we observed a case of scleromyxedema ...

Enalaprilat (Ena.), an angiotensin II (Ang II) converting enzyme inhibitor (ACEI), can produce some therapeutic effects on hypertension, ventricular hypertrophy and myocardial remodeling in clinic, but its precise mechanism, especially its signaling pathways remain elusive. In this study, cardiac fibroblasts (CFb) was isolated by the trypsin digestion method; a BrdU proliferation assay was adop...

evaluation of autogenous fibrinogen extracted from horse blood in fibrin glue and it's effects on horse's fibroblast and keratinocyte cells were preformed. fibrinogen has been obtained by glycine precipitation technique from horse's blood. fibroblast cells were separated by explant culture method from neck skin and keratinocytes were separated by enzyme digestion from lib samples...

Objective: The purpose of this research is to assess the antioxidant activity lemongrass leaves extract in terms lowering ROS generation and its effect on viability proliferation fibroblasts under oxidative stress. Material Methods: was measured using DPPH method assay carried out by fluorescent H2DCFDA staining. Viability assays were performed Cell Counting Kit-8 (CCK-8) read at 450 nm micropl...