نتایج جستجو برای: fluorometric assay
تعداد نتایج: 223370 فیلتر نتایج به سال:
Histamine (Hm), formed during decarboxylation of L-histidine by the action of L-histidine decarboxylase, a pyridoxal phophate dependent enzyme, is one of the main mediator in such allergic diseases as asthma, urticaria and hay fever. Hence Hm assay can be used as a diagnostic tool in these disorders. There are three main methods for Hm assaying: (1) radioenzymatic (2) radioimmunoassay (RIA) ...
A fluorometric Fast Analyzer has been developed, based on the GeMSAEC Fast Analyzer principle. Potential applications of a multiple-cuvet fluorometric analyzer are considered. A primary concern must be referencing enzyme activity assays and enzymic substrate analyses. Once adequate referencing techniques are developed, adaptation of analyses can proceed. The advantages of fluorescence procedure...
We have developed a straightforward fluorometric assay to measure primase-polymerase activity of human PrimPol (HsPrimPol). The sensitivity of this procedure uncovered a novel RNA-dependent DNA priming-polymerization activity (RdDP) of this enzyme. In an attempt to enhance HsPrimPol RdDP activity, we constructed a smart mutant library guided by prior sequence-function analysis, and tested this ...
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