The advent of 3D in vitro organoids that mimic the in vivo tissue architecture and morphogenesis has greatly advanced the ability to study key biological questions in cell and developmental biology. In addition, organoids together with recent technical advances in gene editing and viral gene delivery promises to advance medical research and development of new drugs for treatment of diseases. Or...

The rate at which the transmitter release machinery is fixed by 2% glutaraldehyde at hippocampal synapses and the amount of release evoked by the fixative have been investigated. We recorded from hippocampal cells while fixative was applied with a rapid flow system. Release is blocked in less than a second and fixative-produced exocytosis is at most a few percent of what would be caused by a hy...

IN studying the distribution in tissues of substances which react with reduced fuchsin, it is frequently necessary to distinguish between (a) substances which react as though they were free aldehydes; (A) substances which react as though they were aldehyde acetals; and (c) substances which react as aldehydes as a result of oxidation of precursors in the tissue. In a recent paper on the plasmal ...

Raman spectroscopy is a non-invasive and non-destructive tool that has been widely applied in Agricultural and Bio-system Engineering field. It provides a unique “fingerprint” to different chemical, and structural, information at molecular level. Acquisition of Raman spectrum requires a relatively short collecting time and minimum sample preparation, which makes Raman spectroscopy an attractive...

Background Sample preparation is the first step in the evaluation of tissue samples. In this research tried to using two types of formalin and Bouin fixative ovarian tissue morphogenesis be evaluated practical. MaterialsAndMethods Ovaries of 6-8 week-old Naval Medical Research Institute female mice were randomly divided into 3 groups: Ι. Ovaries that immediately fixed in Bouin’s fixative; II. O...

A quicker alternative to the standard removal and fixation of brain tissue was sought. Whole fetal brains were fixed in situ using a mercuric based fixative. The subarachnoid space was perfused overnight with Heidenhain's Susa fixative. The following day the brains were removed from the cranium in the standard manner. After storage for three days in Susa's fixative the brain was sliced and proc...

background: human hair can be used as an inexpensive and accessible adsorbent to remove a variety of pollutants from air. although several studies have been done on removal of formaldehyde from wastewater by human hair, to date no study has investigated using hair to remove formaldehyde from air. therefore, the aim of this study was to remove formaldehyde from synthetic contaminated air by a re...