DNA is susceptible of being damaged by chemicals, UV light or gamma irradiation. Nuclear DNA damage invokes both a checkpoint and a repair response. By contrast, little is known about the cellular response to mitochondrial DNA damage. We designed an experimental system that allows organelle-specific DNA damage targeting in Saccharomyces cerevisiae. DNA damage is mediated by a toxic topoisomeras...

Damage tolerance mechanisms mediating damage-bypass and gap-filling are crucial for genome integrity. A major damage tolerance pathway involves recombination and is referred to as template switch. Template switch intermediates were visualized by 2D gel electrophoresis in the proximity of replication forks as X-shaped structures involving sister chromatid junctions. The homologous recombination ...

Scale formation and well plugging due to the incompatibility of injected waters is a critical field problem in oil recovery. A water injection process was successfully performed when the identification of the source of water, water quality considerations, and laboratory compatibility tests were considered. When different waters are mixed, it is necessary to evaluate their compatibility prior to...