Ramon Crehuet,† Josep M. Anglada,*,† Dieter Cremer,*,‡ and Josep M. Bofill§ Departament de Quı́mica Orgànica Biològica, Institut d'InVestigacions Quı́miques i Ambientals de Barcelona, CSIC, C/ Jordi Girona 18, E-08034 Barcelona, Catalunya, Spain, Department of Theoretical Chemistry, UniVersity of Göteborg, Reutersgatan 2, S-41320, Göteborg, Sweden, and Departament de Quı́mica Orgànica and Centre d...

Runx1 participation in epithelial mammary cells is still under review. Emerging data indicates that Runx1 could be relevant for breast tumor promotion. However, to date no studies have specifically evaluated the functional contribution of Runx1 to control gene expression in mammary epithelial tumor cells. It has been described that Runx1 activity is defined by protein context interaction. Inter...

Unexplained recurrent spontaneous abortion (URSA) is an alloimmune disease associated with the failure of fetal-maternal immunologic tolerance in which the regulatory T lymphocytes (Treg) play a pivotal role. It is well known that Forkhead box P3 (Foxp3) is a crucial regulatory factor for the development and function of Treg cells. It has also been established that deficiency of the Foxp3 gene ...

Impaired functional activity of T regulatory cells has been reported in allergic patients and results in an increased susceptibility to autoimmune diseases. The master regulator of T regulatory cell differentiation, the transcription factor FOXP3, is required for both their development and function. Despite its key role, relatively little is known about the molecular mechanisms regulating foxp3...

Homeostasis in the immune system is maintained by specialized regulatory CD4(+) T cells (T(reg)) expressing transcription factor Foxp3. According to the current paradigm, high-affinity interactions between TCRs and class II MHC-peptide complexes in thymus "instruct" developing thymocytes to up-regulate Foxp3 and become T(reg) cells. However, the loss or down-regulation of Foxp3 does not disrupt...

Dogma that the regulatory T cell (Treg) prevents catastrophic autoimmunity throughout the lifespan relies on the assumption that the FoxP3 locus is transcribed exclusively in Treg. To test the assumption, we used the Rag2(-/-) and the Rag2(-/-) mice with the Scurfy (sf) mutation (FoxP3(sf/Y) or FoxP3(sf/sf)) to evaluate FoxP3 expression outside of the lymphoid system. Immunohistochemistry and r...

Abstract Background and Aims Regulatory T cells modulate autoimmune allogenic responses in glomerulonephritis transplantation. The two most abundant fork-head box P3 (FOXP3) splice variants, full-length FOXP3 (FOXP3-FL) lacking exon 2 (FOXP3-Δ 2), confer suppressive ability to regulatory cells. Some studies showed a weaker of FOXP3-Δ 2. Renal organic osmolytes, urea Trimethylamine N-oxide (TMAO...

IL-2 plays a critical role in the maintenance of CD4+CD25+ FOXP3(+) regulatory T cells (Tregs) in vivo. We examined the effects of IL-2 signaling in human Tregs. In vitro, IL-2 selectively up-regulated the expression of FOXP3 in purified CD4+CD25+ T cells but not in CD4+CD25- cells. This regulation involved the binding of STAT3 and STAT5 proteins to a highly conserved STAT-binding site located ...

mit [Ru(,buS5')]-Fragmenten ('buS5,-H 2 = 2,2'-Bis(2-mercapto-3,5-di/-butylphenylthio)diethylsulfid) Transition Metal Complexes with Sulfur Ligands, CXV+. Protonation and Alkylation of Thiolate Donors in Ru(II) Complexes with [Ru('buS5')] Fragments ('buS5'-H 2 = 2,2'-Bis(2-mercapto-3,5-di-t-butylphenylthio)diethylsulfide) Dieter Sellmann*, Christine Rohm, Matthias Moll Institut für Anorganische...

Naturally occurring regulatory T (Treg) cells, which specifically express the transcription factor forkhead box P3 (Foxp3), are engaged in the maintenance of immunological self-tolerance and homeostasis. By transcriptional start site cluster analysis, we assessed here how genome-wide patterns of DNA methylation or Foxp3 binding sites were associated with Treg-specific gene expression. We found ...