When Clostridium tetani was discovered and identified as a Gram-positive anaerobic bacterium of the genus Clostridium, the possibility of turning its toxin into a valuable biological carrier to ameliorate neurodegenerative processes was inconceivable. However, the non-toxic carboxy-terminal fragment of the tetanus toxin heavy chain (fragment C) can be retrogradely transported to the central ner...

Fab/c fragments, purified from pepsin digest of mouse IgG2b monoclonal antibody (MoAb) 791T/36, consist of one Fab arm and the intact Fc portion. Pharmacokinetic and biodistribution studies in BALB/c mice of radioiodine-labeled 791T/36 MoAb and its Fab/c fragments showed that, due to the presence of the Fc portion, the Fab/c fragment has the same catabolic rate as whole antibody (T1/2 = 64 h). ...

The octapeptide 61-68 and the heptapeptide 61-67 do not possess the protected conformation of C-fragment. In contrast with the protracted exposure to membrane enzymes required to form [methioninelenkephalin from C-fragment, the short peptides rapidly undergo this conversion. The corresponding tripeptide Thr-Ser-Glu and dipeptide Thr-Ser were isolated. The results suggest that C-fragment is degr...

Herein, the pyridinophane tetradentate ligand PyNMe 3 is used to isolate and structurally characterize well-defined organometallic Ni( ii ) iii complexes bearing cycloneophyl fragment, an alkyl/aryl C-donor ligand.

We report here on the screening of a fragment library against a G-quadruplex element in the human c-MYC promoter. The ten fragment hits had significant concordance between a biophysical assay, in silico modelling and c-MYC expression inhibition, highlighting the feasibility of applying a fragment-based approach to the targeting of a quadruplex nucleic acid.

The aims of the present study were to propose apolymerase chain reaction-restriction fragment length polymorphismPCR-RFLP genotyping method for the AY_376689:c.773C>T single nucleotide polymorphism SNP of the equine PRKAG3 gene, related to muscle performance, as well as to characterize this SNP and a second polymorphism, AAWR_02017454:g.121684T>C of the SPATA1 gene, related to male fertility, i...