In recent years, data from fluorescence recovery after photobleaching (FRAP) has been analyzed quantitatively to estimate the binding rates of cellular proteins to cellular scaffolds such as chromatin. Unfortunately, the accuracy of these estimates is uncertain, since no benchmarks exist. To address this uncertainty, we have compared three different FRAP analysis procedures that have been appli...

BACKGROUND Salivary markers of oxidative stress and antioxidant status represent promising tool for the research of oral diseases. One of the criteria is the validation of these biomarkers from the perspective of the confounding and modifying factors. AIM To examine the effect of circadian rhythm, tooth-brushing and ascorbic acid treatment on selected salivary markers of oxidative and carbony...

pomegranate, a small tree with potential human health benefits, is grown mainly in iran, india and usa as well as in most near and far east countries. it has been used extensively in folk medicine for a number of therapeutic purposes. in the present study, the ferric reducing/antioxidant power assay (frap) was employed and the frap value of the seed fraction of six different cultivars of pomegr...

Accepted: 08/06/2014 Abstract Six variety of betel leaf from different domain of India were extracted using five solvents in order to determine the effect of solvent and changing variety on total phenol content and antioxidant activity. Antioxidant activity was determined using DPPH, ABTS, FRAP, PCL (Photochemluminiscence) assay. However 80% methanol for TPC, 80% ethanol followed by 80% methano...

Grapes are widely known for health benefits due to their antioxidant content. In wine production, grape stems are often discarded, though they has a higher content of antioxidants than the juice. The effectiveness of using an environmentally friendly solvent, ethanol, as a superheated liquid and supercritical fluid to extract antioxidant compounds from grape stems of organically grown Crimson S...

Fluorescence recovery after photobleaching (FRAP) has become a popular technique to investigate the behavior of proteins in living cells. Although the technique is relatively old, its application to studying endogenous intracellular proteins in living cells is relatively recent and is a consequence of the newly developed fluorescent protein-based living cell protein tags. This is particularly t...