Atthough it is generally believed that under natural conditionso freeze-tolerant organisms can survive only if ice formation is restricted to the extracellular space, in 1959 R. W. Salt reported that fat body cefls of the freeze-tolerant gall fly, Eurosta solidaginis (Diptera: Tephritidae)' survive intracellular freezing. Using cryomicroscopy, intratellular freezing was observed at -4.6*0.1oC f...

This paper investigates the thermo-hydro-mechanical (THM) behaviour of soils subjected to seasonal temperature variations in both permafrost and seasonally frozen conditions. Numerical modelling of soil freezing and ice segregation processes is presented, and compared against small-scale physical modelling experiments. The coupled THM model presented, which is solved by way of a transient finit...

The freezing stage governs several critical parameters of the freeze drying process and the quality of the resulting lyophilized products. This paper presents an integrated ultrasonic transducer (UT) in a stainless steel bottle and its application to real-time diagnostics of the water freezing process. The sensor was directly deposited onto the stainless steel bottle using a sol-gel spray techn...

SENSITIVE TO FREEZING2 (SFR2) is crucial for protecting chloroplast membranes following freezing in Arabidopsis (Arabidopsis thaliana). It has been shown that SFR2 homologs are present in all land plants, including freezing-sensitive species, raising the question of SFR2 function beyond freezing tolerance. Similar to freezing, salt and drought can cause dehydration. Thus, it is hypothesized tha...

BACKGROUND Induced sputum (IS) analysis is a noninvasive, valid, and reproducible method for evaluating airway inflammation. It has been suggested that freezing of IS samples in order to delay analysis is feasible. However, the optimal conditions for preservation of IS samples have not been determined. OBJECTIVES To determine optimal freezing conditions of IS samples, ensuring adequate specim...

The fact that freezing caused marked changes in oxidative activity of tissues was of comparatively little interest until it became apparent that most if not all the enzymatic activities of cells could be reproduced under suitable conditions in cell-free systems. Isolated soluble enzymes were usually unaffect,ed or even stabilized by freezing, and damaging effects of freezing on other enzymes co...

This study aims at providing new insight on protein denaturation in freezing-thawing. Freezing-thawing minced pork reduced water-holding of myofibrils and increased surface hydrophobicity. One additional freezing-thawing cycle slow freezing rate caused appearance a 160 kDa myosin-4 fragment SDS-PAGE, further decreased Fresh was exposed to either high salt (2 M KCl) only or with lower pH mimic c...

damage caused by freezing can be the result of differences in cellular processes, including changes in membrane properties, interactions between macromolecular and enzymatic reactions. in this experiment, total soluble solids (tss) and relative water content of the reproductive buds of cultivars “j. h. hale”, “elberta,” “redhaven, ” “sorkh sefid mashhad” and “amsden” peaches, along with nectari...