One hundred forty-four microorganisms previously isolated from coffee fruit (Coffea arabica) were grown on casein agar to evaluate their proteolytic activities. Fifty percent of filamentous fungi, 52.5% of bacteria and 2.6% of yeasts were able to secrete proteases. Positive isolates were further examined in liquid culture for their protease activities by hydrolysis of casein at different pH val...

The seed-borne mycoflora of sorghum and foxtail millet collected from different growing areas in South Korea were isolated and taxonomically identified using dry inspection, standard blotter and the agar plate method. We investigated the in vitro and in vivo germination rates of disinfected and non-disinfected seeds of sorghum and foxtail millet using sterilized and unsterilized soil. The perce...

Four water-soluble Fusarium metabolites (fumonisin B1, fusaric acid, butenolide and moniliformin), water-insoluble pigment (8-O-methylbostrycoidin), and an Alternaria metabolite (AAL-toxin) were tested for relative cytotoxicity to five established mammalian cell lines. Butenolide was the most cytotoxic to all five cell lines. LC50s were; 1 microgram/ml to rat hepatoma (RH) (tumors derived from ...

Each of two horses was dosed by stomach tube with culture material on maize of Fusarium moniliforme MRC 826. One horse developed severe hepatosis and mild oedema of the brain after 6 doses of 2.5 g of culture material/kg body mass/day in 7 days. The second horse, in a similar experiment but at a dosage rate of 1.25 g/kg/day, developed mild hepatosis and moderate oedema of the brain. In both ani...

Enzymatic activity e.g., pectinase, cellulase, protease and lipase of different fungal pathogens causing stalk rot disease in corn was determined. In-vitro studies; corn stalk rot pathogens viz., Cephalosporium acremonium, Fusarium moniliforme, F. graminearum, F. semitectum, Macrophomina phaseolina, st. 1, M. phaseolina st. 2, Rhizoctonia solani st.1, R. solani st. 2 and Verticillium albo-atrum...

Chrysanthemum morifolium is one of the most famous cut flowers with a high ornamental value, occupying an irreplaceable position in international flower commerce. But frequently occurred fungal diseases limit production this plant. A total five fungicides viz., CM-75 WP, Dithane M 45, Ridomil Gold MZ 68 WG, Rovral 50 WP and Score 250 EC were selected to evaluate vitro efficacy at 100, 200, 300,...

We have investigated any permanent impact on development stages of Drosophila melanogaster in this study. Thus F1 and F2 generations of Drosophila melanogaster, obtained from parents that were fed in bottles containing SDM with test compounds of Fumonisin B1 chronically, were checked in terms of developmental stages of Drosophila melanogaster. But any effect has not been found. In addition to t...

During present investigation, bacterial strains were isolated from rhizosphere of healthy and infected fields of maize grown at Yousafwalla, Pakistan. Fourteen isolates were screened for their antibacterial and antifungal activities. Three isolates showed the antimicrobial activity against Pseudomonas aeruginosa and Bacillus subtilis. Staphylococcus aureus and Klebsiella pneumoniae were found t...