A galactomannan-protein complex was extracted from defatted mycelium of Histoplasma capsulatum with 0.25 N alkali at 25 C. It accounted for 7.5% of the cell dry weight in nondialyzable form and had a galactose-mannose ratio of 2:5. The complex containing 68% protein was separated into polysaccharide and glycoprotein components by ion-exchange chromatography. The galactomannan formed a single pr...

BACKGROUND AND AIMS Galactomannans act as storage reserves for the seeds in some plants, such as guar (Cyamopsis tetragonoloba) and coffee (Coffea arabica and Coffea canephora). In coffee, the galactomannans can represent up to 25 % of the mass of the mature green coffee grain, and they exert a significant influence on the production of different types of coffee products. The objective of the c...

Invasive aspergillosis is one the major causes of death in hematopoietic and solidtransplant recipients. One of the most critical problems in medical mycology is the inability to consistently make a convincing and early diagnosis of invasive fungal infection. Conventional diagnosis methods such as culture-based approaches are limited by the insufficient accessibility sensitivity and the non-cul...

Two diagnostic tests, an Aspergillus-specific PCR and an enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of galactomannan, were compared for diagnosing and monitoring invasive pulmonary aspergillosis. Persistently neutropenic rats with left-sided invasive pulmonary aspergillosis were sacrificed at regular intervals after inoculation. Blood samples and bronchoalveola...

The role of Aspergillus fumigatus in the airways of chronic obstructive pulmonary disease (COPD) patients with bronchiectasis is currently unclear. We searched for a sensitive and noninvasive method for A. fumigatus detection in the sputum of COPD patients and addressed potential risk factors for its presence. Induced sputum samples of 18 COPD patients and 17 COPD patients with bronchiectasis w...

Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structural studies of the galactomannan of Aspergillus fumigatus, some inconsistencies ...

Cereal arabinoxylans, guar galactomannans, and dextrans produced by lactic acid bacteria (LAB) are a structurally diverse group of branched polysaccharides with nutritional and industrial functions. In this thesis, the effect of the chemical structure on the dilute solution properties of these polysaccharides was investigated using size-exclusion chromatography (SEC) and asymmetric flow field-f...

Detached inflorescences of guar (Cyamopsis tetragonoloba), each bearing 4 uniformly-developing pods at 42 days post anthesis (DPA), were cultured for 6 days in complete liquid medium manipulated with a fixed concentration of mannose and varying concentration of myo-inositol. Such inflorescences, but with 2 pods, were also maintained in the solutions of (i) glucose(U-14C) containing myo-inositol...

Invasive aspergillosis is one the major causes of death in hematopoietic and solidtransplantrecipients. One of the most critical problems in medical mycology is theinability to consistently make a convincing and early diagnosis of invasive fungalinfection. Conventional diagnosis methods such as culture-based approaches are limitedby the insufficient accessibility sensitivity and the non-culture...

A water soluble galactomannan having D-Mannose [4-part] and D-Galactose [2-parts] was isolated from Cassia fistula. In cassia fistula Hydrolysis of methylated seed gum furnished three methylated sugars, 2, 3-di-O-methyl-D-mannose, 2, 3,6-tri-O-methyl-D-mannose and 2, 3,4,6-tetra-Omethy-D-galactose was present in molar ratio 3:2:1. Partial acid catalysed hydrolysis of the seed gum gave five olig...