The endogenous beta-D-galactoside-binding lectins of UV-2237-IP3 fibrosarcoma cells consist of two polypeptides with molecular weights of 14,500 (L-14.5) and 34,000 (L-34). Recently we constructed complementary DNA (cDNA) libraries from these cells in the expression vector lambda gt11 and isolated and characterized cDNA clones encoding the two lectin species (A. Raz et al., Exp. Cell Res., 173:...

In this report, we challenge a common perception that tumor embolism is a size-limited event of mechanical arrest, occurring in the first capillary bed encountered by blood-borne metastatic cells. We tested the hypothesis that mechanical entrapment alone, in the absence of tumor cell adhesion to blood vessel walls, is not sufficient for metastatic cell arrest in target organ microvasculature. T...

Cholesterol-derivatized galactosides have been devised in order to induce liver uptake of lipoproteins via the galactose-recognizing asialoglycoprotein receptor in the liver. In this study we describe the derivatization of a newly developed triantennary cluster galactoside having high affinity for the asialoglycoprotein receptor, N-[[tris-O-(3,6,9-trioxaundecanyl-beta-D-galactopyranosyl)metho x...

The purpose of this research was to identify amino acid residues that mediate substrate recognition in the lactose carrier of Escherichia coli. The lactose carrier transports the alpha-galactoside sugar melibiose as well as the beta-galactoside sugar lactose. Mutants from cells containing the lac genes on an F factor were selected by the ability to grow on succinate in the presence of the toxic...

High-sensitivity isothermal titration calorimetry has been used to investigate the thermodynamics of binding of Ricinus communis agglutinin to galactose, lactose and their derivatives in the temperature range 280.5-298 K. The present study unequivocally establishes the carbohydrate-binding stoichiometry of the tetrameric agglutinin from castor bean as two, i.e. the (As-sB)2-type tetramer of the...

Lactose and o-nitrophenylgalactoside (NPG) were transported against considerable concentration gradients by ML 308-225, a mutant of Escherichia coli which lacks /3-galactosidase but possesses a constitutive transport system for P-galactosides. The kinetics of influx of NPG during this accumulation were found to be the same as those found for this galactoside moving down a concentration gradient...

The distribution of endogenous beta-galactoside-specific lectin, fibronectin, type I and III collagens was studied by the indirect immunofluorescence method during the formation of dermal condensation in the feathered region (dorsal skin) of a chick embryo. Endogenous beta-galactoside specific lectin was concentrated in the condensed dermal region, coinciding with the formation of condensation ...

Blueberries are particularly rich in anthocyanins and favored extensively for their health benefits. In this study, the anthocyanin profiles of fruits four blueberry cultivars (Gardenblue, Legacy, Misty, Brightwell) were investigated. Total content gradually increased all during development, but mature Brightwell Gardenblue had a higher than those Legacy Misty. Thirty-two kinds quantified using...