Cancellous bones from twenty five live tissue donors were tested for bacterial contamination and initial bioburden ranged from 4.1×10 to 3.1×10 cfu/g (average 9.0×10 cfu/g). Forty six representative bacterial isolates were characterized on the basis of morphological, cultural and biochemical characteristics. Staphylococcus spp. was found to be predominant contaminant in tissue samples (41.30%)....

The aim of this study was to determine the feasibility of using high pressure CO2 for sterilization of Ginseng powder, as an alternative method to conventional techniques such as gamma-irradiation and ethylene oxide. The Ginseng sample used in this study was originally contaminated with fungi and 5 x 10(7) bacteria/g that was not suitable for oral use. This is the first time that high pressure ...

Sterilization is a quite challenging step in the development of novel polymeric scaffolds for regenerative medicine since conventional sterilization techniques may significantly alter their morphological and physicochemical properties. Supercritical (sc) sterilization, i.e. use scCO2 as sterilizing agent, emerges promising method due to mild operational conditions excellent penetration capabili...

Cell-assembled extracellular matrix (CAM) has been used to produce vascular grafts. While these completely biological grafts performed well in clinical trials, the vivo remodeling and inflammatory response of this truly “bio” material not yet investigated. In study, human CAM yarns were implanted subcutaneously nude rats investigate innate immune matrix. The impact processing steps relevant yar...

Allograft safety is contingent on effective sterilization methods such as gamma irradiation. However, recent clinical studies have associated gamma irradiation with decreased graft strength and increased allograft failure rates, despite data that shows no deleterious effects to soft tissue biomechanics at gamma doses between 1.46 and 2.18 mRad. The objective of this study was to compare the bio...

In this work, isothermal microcalorimetry (IMC) was utilized to measure the exothermic heat flow from specimens of ultra-high-molecular-weight polyethylene (UHMWPE), that had been sterilized by various standard methods, under simulated shelf storage (air at 25 degrees C, 30% r.h.) and simulated implantation (phosphate buffered saline, PBS, at 37 degrees C) conditions. Gamma-radiation sterilized...

Use of musculoskeletal allografts has become increasingly popular, with widespread use among knee surgeons. The advantages and disadvantages of their use have been documented. In the knee, allografts are used for ligament reconstruction, meniscal transplantation, and articular surface reconstruction. The purpose of this review is to present issues surrounding the allograft industry, including r...