background and aims: the aim of this study was application of gamma irradiated fmdv antigen for liquid phase blocking sandwich elisa (b-elisa) to quantify guinea pig anti fmdv type a87/irn antibody. methods: fmdv type a87/irn was propagated to a titer of 107.5/ml tcid50 and then irradiated by gamma irradiator. the irradiated 146 s antigen was purified by sucrose gradient centrifugation after in...

A sensitive two-step simultaneous enzyme immunoassay (EIA) for human gamma interferon (IFN-gamma) has been developed and used as an in vitro test for human tuberculosis (TB) in comparison with tuberculin skin testing. The EIA was shown to be highly sensitive, detecting less than 0.5 IU of recombinant human IFN-gamma per ml within a linear detection range of 0.5 to 150 IU/ml. The assay was highl...

an extensive study was undertaken to investigate on the overall film badge service performance with which occupational radiation, exposures are reported in iran, the study involved the analysis of film badges exposed to x-and gamma rays and beta particles. for test exposures, x-rays between 60 and 140 kvpco-60 gamma radiation and sr-90 beta particles were used. the actual delivered dose equival...

OBJECTIVE To evaluate an interferon (IFN)-gamma release assay in diagnosing latent tuberculosis infection in pregnant adolescents and women at risk for exposure to Mycobacterium tuberculosis. METHODS This was a prospective study of women and adolescents receiving health care at Bellevue Hospital Outpatient Clinics in New York City. Each patient was assessed for M tuberculosis risk factors, ha...