A method for gas-liquid chromatographic separation and quantitation of urinary steroid metabolites is described. A normal reference range has been established and classic examples of urinary steroid profiles of the major steroid disorders are given.

A microprocessor-controlled automatic extractor, (PREMTM I) is described with which lipophilic components may be extracted from physiological fluids by means of a selective, solid-phase extraction cartridge, and the extracts presented in dry form for subsequent analysis. This instrument has been successfully evaluated for the extraction of phenobarbital, primidone, and phenytoin from human seru...

Concentrations of diphenylhydantoin in 364 plasma samples have been measured both by radioimmunoassay and with a recently developed gas-chromatographic method, which requires only 0.1 ml of plasma per determination. There was an excellent correlation between values obtained by the two methods (r = 0.986), and in only 11 plasma samples did the results differ by more than 20%. Of the investigated...

._----__-_--_-_-______--_----_--_-_----_--__--------_-Bl Introduction-__-____--_-_---_____---_--_---__-_--------_--------__ 1 Collection and handling of samples.-_-----_-__--__-------_-_---__--3 Electron-capture gas chromatographic method-___..---_---,______.___ 4 Apparatus.-__-__---_-----_------------_-------_-_-------__-__ 4 Reagents-_-____----_---______-_---_-_-_-_-_-_-___-_---_-----4 Proced...