A bstract. This paper describes a new method for qualitative and quantitative assay of abscisic acid and other acidic plant hormones, such as indoleacetic acid and the gibberellins, by the gas-liquid chromatography of their trimethylsilyl derivatives. Interfering substances in plant extracts were largely removed by preliminary oolumn chromatography with carbon-celite and elution of the absoisic...

1-Deoxyglucose (1,5-anhydroglucitol), a metabolite related to diabetes mellitus, was identified in human plasma by gas-liquid chromatography and by gas-liquid chromatography/mass spectrometry. Plasma polyols were accurately determined with a gas-liquid chromatograph equipped with an all-glass capillary column. The plasma content of 1-deoxyglucose in healthy persons varies with age. Although the...

A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococ...

separation of ten sedative- hypnotic drugs was performed by rp-hplc using mixed micellar mobile phase. effect of temperature, type and amount of organic modifier in mobile phase on efficiency (n) and asymmetry factor (b/a) showed that, the appropriate conditions for a good separation were 35°c and 7% (v/v) butanol in mobile phase.  variations of selectivity factor versus butanol concentration, ...

In recent years there has been considerable interest in the synthesis and separation of enantiomers of organic compounds especially because of their importance in the biochemistry and pharmaceutical industry. High-performance liquid Chromatography is a very useful method for the direct separation of enantiomers. However, about 30−40 years ago, commercially available chiral stationary phases wer...

Dispersive liquid-liquid microextraction (DLLME) followed by gas chromatography-massspectroscopy (GC-MS) was used to analyses of components of floral-water of Rosmarinusofficinalis . Some effective parameters such as volume of extraction, disperser solvents andsalt effect were studied by a full factorial design for obtain the optimum condition. The maincompounds of the floral-water of Rosmarinu...

A rapid gas-liquid chromatographic procedure was developed to determine hippurate hydrolysis by microorganisms. Bacterial cells were inoculated into 0.4 ml of 1% sodium hippurate and incubated for 2 h at 37°C. Cells were removed by centrifugation, and the benzoate released by enzyme activity was converted to methyl benzoate and analyzed by gas-liquid chromatography. This procedure is sensitive,...

For years, microGC analysis has been useful as a powerful tool for the fast and reliable analyses of natural gas and other gaseous matrices; but, it is yet to prove its capabilities for liquid samples. In this paper, we present a unique gasifying system for volatile liquid sample introduction in a microGC. The system has been tested with a wide range of different samples: liquefied gases (ethan...