Potato (Solanum tuberosum L.) has a tetraploid genome. To make mutant lacking specific gene function, it is necessary to introduce mutations into all four alleles. achieve this goal, we developed powerful genome editing tool, CRISPR/dMac3-Cas9, which installed the translation enhancer dMac3 that greatly increased of downstream open reading frame. The CRISPR/dMac3-Cas9 system employing three gui...

The molecular deposition of starch extracted from normal plants and transgenically modified potato lines was investigated using a combination of light microscopy, environmental scanning electron microscopy (ESEM) and confocal laser scanning microscopy (CLSM). ESEM permitted the detailed (10 nm) topographical analysis of starch granules in their hydrated state. CLSM could reveal internal molar d...

Cereal Chem. X()(4):437-445 Fourteen l10ur blends of two natural wild-type wbeat (71'iticlIllI lIestiVll1n L.) nours, 'Nuplains' and 'Centum', blended with one waxy nour sample were chamcterized and processed to Asian salted noodles. The flour amylose content range was <1-29%. Damaged starch contents were lOA, 7.0, and 6.6% for the waxy wheat, Nuplains, and Centum, respectively. The waxy flour ...

EEG (Electroencephalography) resting state was studied by means of group blind source separation (gBSS), employing a test-retest strategy in two large-sample normative databases (N=57 and N=84). Using a BSS method in the complex Fourier domain and a model-driven distributed inverse solution we closely replicate both the spatial distribution and spectral pattern of seven source components. Norms...

Apparent amylose content (AAC), the key determinant of rice end-use quality attributes, is primarily controlled by the Waxy gene which codes for granule bound starch synthase (GBSS). We examined the combination of sequence variation in the Waxy gene and environmental effects, and their associations with AAC using 171 rice accessions originating from 43 countries. The combination of two single-n...

The effect of food components on degradation of DNA by DNase I (EC 3.1.21.1) was monitored by electrotransformation of Escherichia coil, making it possible to determine the number of plasmid molecules capable of giving rise to transformed cells. The transformation frequency increased linearly with the plasmid number within the range of 2 x 10(6) to 2 x 10(10). DNA degradation was reduced by one...

TALEN is an artificial nuclease being applied for sequence-specific genome editing. For the plant genome editing, a pair of TALEN genes is expressed in the cells, and a binary plasmid for Agrobacterium-mediated transformation should be assembled. We developed a novel procedure using the Gateway-assisted plasmids, named Emerald-Gateway TALEN system. We constructed entry vectors, pPlat plasmids, ...

Due to their limited computation capabilities and battery life, Internet of Things (IoT) networks face significant challenges in executing delay-sensitive computation-intensive mobile applications services. Therefore, the Unmanned Aerial Vehicle (UAV) edge computing (MEC) paradigm offers low latency communication, computation, storage capabilities, which makes it an attractive way mitigate thes...

Starch is an important reserve of carbon and energy in plants, providing the majority of calories in the human diet and animal feed. Its synthesis is orchestrated by several key enzymes, and the amount and structure of starch, affecting crop yield and quality, are determined mainly by starch synthase (SS) activity. To date, five SS isoforms, including SSI-IV and Granule Bound Starch Synthase (G...

We investigated the variations in metabolites associated with quality of rice consumption when exposed to varying nitrogen fertilizer levels, as well regulatory role pivotal within metabolic pathways. This research employed Hongyang 5 subject experimentation, examining at three different levels using non-targeted metabonomic analysis. The findings indicated that overall assessment eating qualit...