Apoptosis is a form of programmed cell death characterized by cytoplasmic condensation, plasma membrane blebbing and nuclear pycnosis, leading to nuclear DNA breakdown into multiples of ∼200 bp oligonucleosomal size fragments. The detection of apoptosis in cultured cells relies heavily on techniques involving the extraction of nuclear DNA and characterization of such oligonucleosomal ladders by...

Affinity precipitation with N,, N,'-adipodihydrazido-bis( N6-carboxymethyl-NAD+) (bis-NAD+) as a means of isolating alcohol dehydrogenase (EC 1.1.1.1) and lactate dehydrogenase (EC 1.1.1.27) has already been reported (Flygare et al., 1983; Beattie et al., 1984). However, affinity precipitation of alcohol dehydrogenase in the presence of bis-NAD+ and pyrazole has been found to give mixed results...

Medium conditioned by human placental tissue was found to stimulate granulocytic and monocytic colony formation by human marrow cells in semisolid agar cultures. The colony-stimulating activity of unfractionated conditioned medium was equivalent to the activity of standard peripheral blood underlayers. Placentas were a reliable source of active material, and one placenta provided enough materia...

A deoxyribonuclease distinct from the previously isolated asparagus ribosome-inactivating proteins, possessing a molecular weight of 30 kDa and requiring a pH of 7.5 for optimum hydrolytic activity toward herring sperm DNA, was isolated from Asparagus officinalis seeds. The isolation procedure involved extraction with saline, (NH(4))(2)SO(4) precipitation, ion-exchange chromatography on DEAE-ce...

A virus-free soluble antigen, obtained by ammonium sulfate precipitation of the supernatant fluids of La Crosse virus-infected BHK-21 cell cultures, was more reactive and more specific than infected suckling mouse brain antigen when compared by immunodiffusion and counterelectrophoresis tests. By complement fixation tests, the antigen was cross-reactive with heterologous California group arbovi...

A protein was isolated from bovine thymus that was shown to accelerate the appearance of hemolysin to sheep erythrocytes in neonatal mice. The isolation procedure consisted of saline homogenization, ammonium sulfate precipitation, and methanol precipitation, followed by chromatography on diethylaminoethyl-cellulose and two chromatographies on Sephadex G-150. The protein was shown to be homogene...