background: treatment of cutaneous leishmaniasis (cl) is being faced with serious difficulties in fars province, due to emerging of resistance against meglumine antimonite (glucantime®). in this context, determining some biomarkers for drug sensitivity monitoring seems to be highly essential. different studies have been carried out to decipher the genes might be involved in antimony resistant p...

background and objectives: phosphlipases are a group of enzymes that breakdown phosphatidylcholine (phospholipids) molecules producing second products. these produced products have a divers role in the cell like signal transduction and digestion in humans. in this research the effect of phosphatidylcholine on the expression of plc genes of a. fumigatus was studied. the plc genes of this fungus ...

Non-steroidal anti-inflammatory drugs (NSAIDs) identified effective in many diseases. One of which is neurodegenerative diseases including Alzheimer disease (AD). In this study gross alteration of gene expression in AD mice by ibuprofen treatment is investigated via Protein-protein interaction network (PPI) analysis. Expression profiling of microarray dataset GSE67306 was retrieved from GEO dat...

objective(s): the purpose of this study was to conduct a meta-analysis on human micrornas (mirnas) expression data of endometriosis tissue profiles versus those of normal controls and to identify novel putative diagnostic markers. materials andmethods: pubmed, embase, web of science, ovid medline were used to search for endometriosis mirna expression profiling studies of endometriosis. the mirn...

Background Bone marrow and adipose tissue are two main sources of mesenchymal stem cells (MSCs). Some of studies suggest that there are some differences in gene expression profile of MSCs-derived from various tissues. To investigate gene expression profile by qRT-PCR, an appropriate reference gene with stable expression level should be chosen for normalizing data.  This study was designed to e...

background bone marrow and adipose tissue are two main sources of mesenchymal stem cells (mscs). some of studies suggest that there are some differences in gene expression profile of mscs-derived from various tissues. to investigate gene expression profile by qrt-pcr, an appropriate reference gene with stable expression level should be chosen for normalizing data.  this study was designed to ev...

Background Although spermatozoa delivers its RNA to oocytes at fertilization, its biological role is not well characterized. Our purpose was to identify the genes differentially and exclusively expressed in sperm samples both before and after the swim-up process in control donors and infertile males with the purpose to identify their functional significance in male fertility. MaterialsAndMethod...

objective: the aim of this study was to construct a pcdna3.1+ vector containing fmdv type o/irn/1/2007-vp1 gene, protein expression in bhkt7 cells and evaluation of immune response in balb/c mice. materials and methods: fmdv type o/irn/1/2007 was isolated from a cattle in ray in 2007 and serotyped. the purified vp1 gene was sub-cloned into the ptz57r/t vector and pcdna3.1+ expression vector. t...

The aims of this study were to compare the promoter sequence of the mannose-binding lectin (cMBL) gene in Iranian native and commercial chicken strains; as well as to compare the cMBL gene expression in crossbred and inbred chickens. In total 79 native (Western Azerbaijan native fowls, WANF) and 49 commercial (Arian Commercial Strain, ACS) birds were reared as parents under same management prac...

introduction: g-proteins have an important role in the cell signaling of numerous receptors. the situation of g-proteins in health and disease and their critical role in the development of diabetic side effects is an interested scientific field. here, the changes in the expression of g-protein subunits (gαi, gαs and gβ) were evaluated in hyperglycemic situation of pc12 cells as a cellular model...