Full exploitation of the information available in bacterial genome sequences requires the availability of facile tools for rapid genetic manipulation. One bacterium for which new genetic tools are needed is the methylotroph Methylobacterium extorquens AM1. IncQ and small IncP vectors were shown to be unsuitable for use in this bacterium, but a spontaneous mutant of a small IncP plasmid was isol...

multi-objective optimization is the simultaneous consideration of two or more objective functions that are completely or partially inconflict with each other. the optimality of such optimizations is largely defined through the pareto optimality. multiple objective integer linear programs (moilp) are special cases of multiple criteria decision making problems. numerous algorithms have been desig...