this study aimed to examine the relationship between pigments and seed fall rate of iranian castor genotypes and genetic diversity, specifically 28 castor genotypes (27 native and 1 modified), a randomized complete block design with three replications was conducted in 2014 and 2015 at the research farm of islamic azad university in damghan, northern iran. during the growth period, the following...

cold stress is one of the most important environmental stresses for rice especially in temperate and high elevation areas. in present study, the effect of different temperatures, as the main plot, including 25 (control), 10 and 5°c on 12 rice cultivars (including champa yasouj, gerde, lenjan askari, kaamfirouz, kohsar, shafagh, domsiya mamassani, dular, gharib, hasan saraei, mosa tarom and 304)...

background: cryptosporidium parvum is a zoonotic pathogen transmissible from a variety of animals to humans and is a considerable public health concern. dairy cattle have been identified in numerous reports as a major source of environmen-tal contamination with this pathogen. the aim of study was to detect and isolate the cryptosporidium spp . from fecal samples of naturally infected pre-wean c...

Ribotyping and virulence markers has been used to investigate 68 Yersinia pseudotuberculosis strains of serogroups O:1a and O:3. The strains were isolated from clinical material obtained from healthy and sick animals in the Southern region of Brazil. Ribotypes were identified by double digestion of extracted DNA with the restriction endonucleases SmaI and PstI, separation by electrophoresis and...

Environmental parameters drive phenotypic and genotypic frequency variations in microbial communities and thus control the extent and structure of microbial diversity. We tested the extent to which microbial community composition changes are controlled by shifting physiochemical properties within a hypersaline lagoon. We sequenced four sediment metagenomes from the Coorong, South Australia from...

Three Malaysian ginger cultivars (Bukit Tinggi, Tanjung Sepat and Sabah) were collected and examined for genetic polymorphisms using microsatellite DNA primers. The single microsatellite oligonucleotide primers (CATA)5, (GATA)5 and (GAC)6 were used in polymerase chain reactions (PCRs). These PCR reactions produced 7 polymorphic bands with an average of 2.334 polymorphic bands per primer, leadin...

A novel, Gram-staining-positive, facultatively anaerobic, non-motile and coccus-shaped bacterium, strain WL80(T), was isolated from the gut of an abalone, Haliotis discus hannai, collected from the northern coast of Jeju in Korea. Optimal growth occurred at 30 °C, pH 7-8 and with 1% (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that strain WL80(T) fell within th...

Two novel spore-forming, Gram-positive, mesophilic, heterotrophic bacteria representing two novel species were isolated from the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) at Pasadena, CA, USA. The incidence of similar strains was examined by screening the growing collection of isolates ( approximately 400 strains) obtained from the JPL-SAF using species-specific PCR prime...

Restriction fragment length polymorphisms (RFLP) of the intergenic spacer region (IGS) of rDNA and random amplified polymorphic DNA (RAPD) markers were used to survey genetic variability among 181 isolates of Sclerotinia homoeocarpa from Ontario and 10 isolates from Japan. RAPD and IGS-RFLP analyses revealed polymorphisms within and between populations of S. homoeocarpa, distinguishing 151 geno...

The seagrass Cymodocea nodosa forms a unique community in the Canary Islands, where it is classified as an endangered species. Biogeographic theory predicts that clonal species on islands near their distributional limits might show lower proportions of sexual (versus clonal) reproduction, lower genetic diversity, and higher differentiation. We addressed these hypotheses by comparing the genetic...