A cDNA clone encoding an endo-1,4-beta-glucanase from a rumen fungus, Neocallimastix frontalis MCH3, was isolated. The nucleotide sequence showed that the gene, celA, encoded a multidomain enzyme containing a family 5 catalytic domain and a reiterated sequence that is involved in the association of a multienzyme complex, the cellulosome. The enzyme expressed in Escherichia coli showed the highe...

In 2 experiments, dose response efficacy of a xylanase and β-glucanase blend (XB) on growth performance and ileal nutrient digestibility was investigated in nursery pigs fed pelleted wheat (Triticum aestivum)- and barley (Hordeum vulgare)-based diets. A basal diet (meeting NRC [1998] specifications for 6 to 30 kg BW, except for 5% lower DE) was supplemented with XB to give 4 diets (0, 50, 100, ...

Marine bacteria residing on local red, green, and brown seaweeds were screened for exo-1,3-β-glucanase (ExoP) activity. Of the 90 bacterial species isolated from 32 seaweeds, only one, a Pseudoalteromonas sp., was found to display such activity. It was isolated from a Durvillaea sp., a brown kelp known to contain significant amounts of the storage polysaccharide laminarin (1,3-β-D-glucan with s...

This study was done to determine the effectiveness of supplementary enzymes at increasing the fiber digestion by ruminal microorganisms and to assess whether enzyme activity limits the rate of fiber digestion in ruminal digesta. In vitro comparisons of enzyme activities in two feed enzyme preparations (A and B) with enzyme activities extracted from ruminal fluid indicated that the addition of f...

BACKGROUND The complete degradation of the cellulose requires the synergistic action of endo-β-glucanase, exo-β-glucanase, and β-glucosidase. But endo-β-glucanase and exo-β-glucanase can be recovered by solid-liquid separation in cellulose hydrolysis by their cellulose binding domain (CBD), however, the β-glucosidases cannot be recovered because of most β-glucosidases without the CBD, so additi...

The oomycete organism, Pythium insidiosum, is the etiologic agent of the life-threatening infectious disease called "pythiosis". Diagnosis and treatment of pythiosis is difficult and challenging. Novel methods for early diagnosis and effective treatment are urgently needed. Recently, we reported a 74-kDa immunodominant protein of P. insidiosum, which could be a diagnostic target, vaccine candid...

Inoculation of mature leaves of turnip (Brassica campestris) with the incompatible Xanthomonas campestris pv vitians resulted in the induction of beta-1,3-glucanase and chitinase/lysozyme (CHL) activity. No increase in the basal activity of beta-1,3-glucanase was observed after inoculation of leaves with heat- or rifampicin-killed X. c. vitians, Escherichia coli, or sterile water. Inoculation w...

this study was performed with growing chicken (14 to 56 d of age) to evaluate the effects of feeding them a barley or triticale-based diet. the treatments were corn diet (1) as a control, hulless barley diet with (5) or without (2) enzyme, triticale diet with (6) or without (3) enzyme and hulled barley diet with (7) or without (4) a commercial β-glucanase enzyme. in a digestibility trial, 21 ma...

understanding the function of trichoderma species in the control of phytophthora drechsleri in pistachio orchards is very important.  in this study, the effects of liquid extra-cellular secretions and volatile compounds secreted by 27 isolates of trichoderma harzianum, t. crassum, t. koningii, t.aureoviride, t. asperellum, t. brevicompactum, t.longibrachiatum and t. virens were investigated on ...