In this communication, we present a nanoscale reactor assembled from tuneable and spatially addressable tubular DNA origami units. We can controllably combine separate origami units equipped with glucose oxidase (GOx) and horseradish peroxidase (HRP), and demonstrate efficient GOx/HRP enzyme cascade reaction inside the tube. The reactor could be utilized as a nanoscale diagnostic tool, and modu...

The present work demonstrates the fabrication and performance of an enzymatic glucose biosensor based on ZnO nanowires (NWs) deposited on a Au-coated polyester (PET) substrate. Electrodeposition of ZnO NWs on the conducting PET substrate was carried out at 70 degrees C in an aqueous electrolyte consisting of zinc nitrate mixed with potassium chloride. Glucose oxidase (GOx) was subsequently immo...

Clinical management of diabetes must overcome the challenge of in vivo glucose sensors exhibiting lifetimes of only a few days. Limited sensor life originates from compromised enzyme stability of the sensing enzyme. Sensing enzymes degrade in the presence of low molecular weight materials (LMWM) and hydrogen peroxide in vivo. Sensing enzymes could be made to withstand these degradative effects ...

Three innovative assays are developed for the detection of enzymatic activities of glucose oxidase (GOx) and horseradish peroxidase (HRP) by the generation of CdS quantum dots (QDs) in situ using non-conventional enzymatic reactions. In the first assay, GOx catalyzes the oxidation of 1-thio-β-D-glucose to give 1-thio-β-D-gluconic acid. The latter is spontaneously hydrolyzed to β-D-gluconic acid...

We have proposed a novel free-standing nitrogen-doped carbon nanospheres@carbon nanofibers (NCNSs@CNFs) composite film with high processability for the investigation of the direct electron transfer (DET) of glucose oxidase (GOx) and the DET-based glucose biosensing. The composites were simply prepared by controlled thermal treatment of electrospun polypyrrole nanospheres doped polyacrylonitrile...

Hydrogen peroxide production by glucose oxidase (GOx) and its negative effect on laccase performance have been studied. Simultaneously, FAD-dependent glucose dehydrogenase (FAD-GDH), an O2-insensitive enzyme, has been evaluated as a substitute. Experiments focused on determining the effect of the side reaction of GOx between its natural electron acceptor O2 (consumed) and hydrogen peroxide (pro...

: In this work, molybdenum disulfide (MoS2) nanorods (NRs) were prepared by a simple hydrothermal method. A sensitive electrochemical glucose biosensor was developed based on the immobilization of oxidase (GOx) MoS2 NRs modified glassy carbon electrode (GCE). The SnO2 characterized X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission (TEM), and energy dispersive spectroscop...

In recent years, glucose oxidase (GOx) has aroused great research interest in the treatment of diseases related to abnormal metabolisms like cancer and diabetes. However, as a kind endogenous oxido-reductase, GOx suffers from poor stability system toxicity vivo. order overcome this bottleneck, is encapsulated calcium-based biomaterials (CaXs) such calcium phosphate (CaP) carbonate (CaCO3) by us...

Amperometric biosensors consisting of oxidase and peroxidase have attracted great attention because of their wide application. The current work demonstrates a novel approach to construct an enzymatic biosensor based on TiO2 nanotube arrays (TiNTs) as a supporting electrode on which Prussian Blue (PB)-an "artificial enzyme peroxidase" and enzyme glucose oxidase (GOx) have been immobilized. For t...

Control of glucose concentration has tremendous significance in medical diagnosis, pharmaceuticals, food, and fermentation industries. Herein, we report on the fabrication a facile, low-cost, sensitive enzyme-based amperometric sensor using electrochemically deposited polyaniline (PANI) film graphite electrode. PANI was from an aqueous solution 0.2 M aniline 1.0 hydrocholoric acid (HCl) by cycl...