A new aristolactam, aristolactam 9-O-β-D-glucopyranosyl-(1→2)-β-D-glucoside, and two alkamides, N-cis- and N-trans-p-coumaroyl-3-O-methyldopamine, were isolated from stems of Aristolochia gigantea, together with the known compounds allantoin, E-nerolidol, β-sitosterol, (+)-kobusin, (+)-eudesmin, trans-N-feruloyltyramine, trans-N-coumaroyltyramine, trans-N-feruloyl-3-O-methyldopamine, aristolact...

Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition o...

The Fusarium mycotoxin zearalenone (ZEN) can be conjugated with polar molecules, like sugars or sulfates, by plants and fungi. To date, the fate of these modified forms of ZEN has not yet been elucidated in animals. In order to investigate whether ZEN conjugates contribute to the total ZEN exposure of an individual, ZEN (10 µg/kg b.w.) and equimolar amounts of two of its plant metabolites (ZEN-...

previously, we reported that the kaempferol and kaempferol-7- o -glucoside isolated from securigera securidaca showed potent anti-hsv activity. in the present study the anti-hiv-1 activities of kaempferol and kaempferol-7- o -glucoside are investigated at different concentrations (100, 50, 25 and 10 µg/ml) using hiv-1 p24 antigen kit. real-time polymerase chain reaction (rt-pcr) assay was also ...

sunburn damage is a physiological disorder, which reducing pomegranate fruits quality and result to huge loss to the gardeners yearly. in this study, the effects of kaolin on maintaining fruit quality and decreasing sunburn damage of malas-e-torsh-e-saveh pomegranate was investigated. processed kaolin (surround® wp) with 0, 2.5, 5 and 10 % (w/v) was sprayed over the whole canopy and fruits at 4...

Zearalenone (ZEN) is an estrogenic mycotoxin occurring in Fusarium-infected cereals. Glucosylation is an important plant defense mechanism and generally reduces the acute toxicity of mycotoxins to humans and animals. Toxicological information about ZEN-glucosides is limited due to the unavailability of larger amounts required for animal studies. HvUGT14077, a recently-validated ZEN-conjugating ...

Phytochemical and bioactivity studies of the flowers of Melastoma malabathricum L. (Melastomataceae) have been carried out. The ethyl acetate extract yielded three compounds, identified as naringenin, kaempferol and kaempferol-3-O-d-glucoside, and methanol extract gave kaempferol-3-O-(2′′,6′′-di-O-p-trans-coumaroyl)glucoside and kaempferol-3-O-d-glucoside. The crude extracts and isolated compou...

Expression of the Arabidopsis thaliana MYB transcription factor TRANSPARENT TESTA 2 (TT2) in Medicago trunculata hairy roots induces both proanthocyanidin accumulation and the ATP-dependent vacuolar/vesicular uptake of epicatechin 3'-O-glucoside; neither process is active in control roots that do, however, possess anthocyanidin 3-O-glucoside vacuolar uptake activity. A vacuolar membrane-localiz...

A glycosyltransferase, YjiC, from Bacillus licheniformis has been used for the modification of the commercially available isoflavonoids genistein, daidzein, biochanin A and formononetin. The in vitro glycosylation reaction, using UDP-α-D-glucose as a donor for the glucose moiety and aforementioned four acceptor molecules, showed the prominent glycosylation at 4' and 7 hydroxyl groups, but not a...

Previous investigations have shown that CY4-labeled transcinnamic acid is rapidly converted to o-coumaric acid P-glucoside by shoots of Melilotus alba (white sweet clover) (1) and Hierochlod odorata (sweet grass) (2). This glucoside is then converted to coumarin (l-3), possibly by way of trans-cis isomerization of the glucoside to coumarinic acid P-glucoside. The small amount of radioactivity f...