One hundred and one young-adult female Sprague-Dawley rats were acclimatized to metabolic cages for 2 days. After that time 24-hour urine was collected at a constant cooling temperature of 0-4 degrees C. After gel filtration the enzyme activities were determined, and the resulting values were used to calculate 24-hour excretions. The following reference ranges (2.5 and 97.5 percentiles) were de...

A microvillar fraction was prepared from human kidney cortex. This fraction was seven to 10 times enriched in aminopeptidases N and A, gamma-glutamyltransferase, dipeptidyl peptidase IV, neutral endopeptidase and alkaline phosphatase. Dipeptidyl peptidase IV activity of human renal microvilli could be inhibited by di-isopropylphosphorofluoridate and neutral endopeptidase activity by phosphorami...

BACKGROUND Recent analyses have confirmed that Hispanic and black non-Hispanic Americans are at an increased risk for death from liver cirrhosis. The reasons for this are unknown. As a common cause of cirrhosis, differing sensitivities to alcohol-related hepatocellular injury may play a role. This study compared racial and ethnic aspartate aminotransferase and gamma-glutamyltransferase level el...

BACKGROUND The standard treatment of chronic hepatitis C is the administration of pegylated interferon α2a or α2b in combination with ribavirin, but adverse effects can be observed, as well as the high cost of this therapy. Therefore, there is interest in understanding the predictors of sustained virologic response, as the gamma glutamyltransferase. OBJECTIVE To evaluate the serum levels of g...

We assessed, in 98 patients with cancer, the diagnostic value of measuring serum alkaline phosphatase, 5'-nucleotidase, gamma-glutamyltransferase, and glutamate dehydrogenase activities as an aid to detection of liver metastases. All four enzymes showed diagnostic value, but 5'-nucleotidase appeared to have the greatest. It showed the lowest false-positive results (7.4%) with the highest predic...

Background & AimsGamma-glutamyltransferase (GGT) is a serum marker of cholestasis. We investigated whether level GGT prognostic for patients with primary biliary cholangitis (PBC).MethodsWe analyzed data from PBC the Global Study Group, comprising 14 centers in Europe and North America. obtained measurements at baseline time points after treatment. used Cox model hazard ratios to evaluate assoc...

We have measured the amounts of different molecular forms of y-glutamyltransferase(EC 2.3.2.2), leucine aminopeptidase (EC 3.4.11.2), and alkalinephosphatase(EC 3.1.3.1) in serum of patientswithdifferenttypes of liverdisease.A highmolecular-mass (greater than 1 000 000 Da) form of yglutamyltransferaseand of each of the other enzymes is present in greatest amounts in patients with jaundice from ...

References 1. Landon M. -Glutamyltransferase in breast milk. Ann Clin Biochem 1975;12:48–50. 2. Patel KP, Rangnekar NR. -Glutamyltransferase activity in human milk. Clin Chem 1982;28: 1724–5. 3. Szasz G. A kinetic photometric method for serum -glutamyl transpeptidase. Clin Chem 1969;15:124–36. 4. Britti D, Massimini G, Peli A, Luciani A, Boari A. Evaluation of serum enzyme activities as predict...