1 The Two-Hybrid System: A Personal View Stanley Fields and Paul L. Bartel .............................................. 3 2 Growth and Maintenance of Yeast Lawrence W. Bergman ................................................................ 9 3 Media Formulations for Various Two-Hybrid Systems Michael Saghbini, Denise Hoekstra, and Jim Gautsch ........ 15 4 Yeast Two-Hybrid Vectors and Strain...

Background: Glutathione S-transferases (GSTs) are detoxifying enzymes that contribute to the glutathione-ascorbic acid (vitamin C) antioxidant cycle. Objective: The objective was to determine whether GST genotypes modify the association between dietary vitamin C and serum ascor-

Glutathione transferase A has been purified from rat liver. The enzyme catalyzes the conjugation of glutathione with compounds bearing an electrophilic site, especially those in which the electrophilic site is on, or cr to, an aromatic ring. The enzyme has a molecular weight of 45,000 and is composed of two similar subunits. Initial velocity, product inhibition, and binding studies indicate a b...

The activation of microsomal glutathione S-transferase in oxidative stress was investigated by perfusing isolated rat liver with 1 mM tert-butyl hydroperoxide (t-BuOOH). When the isolated liver was perfused with t-BuOOH for 7 min and 10 min, microsomal, but not cytosolic, glutathione S-transferase activity was increased 1.3-fold and 1.7-fold, respectively, with a concomitant decrease in glutath...

Paracetamol (acetaminophen, PCM) is a widely used over-the-counter analgesic and antipyretic drug. Intake of a large dose of PCM may result in severe hepatic necrosis. Oxidative stress is mediated by oxidative capacities of the PCM metabolite (N-acetyl-para-benzo quinoneimine, NAPQI), which covalently binds to proteins and other macromolecules to cause cellular damage. At low doses, NAPQI is ...

We previously reported that a velvetleaf (Abutilon theophrasti Medic) biotype found in Maryland was resistant to atrazine because of an enhanced capacity to detoxify the herbicide via glutathione conjugation (JW Gronwald, Andersen RN, Yee C [1989] Pestic Biochem Physiol 34: 149-163). The biochemical basis for the enhanced atrazine conjugation capacity in this biotype was examined. Glutathione l...