Lactoferrin (Lf) and folic acid (FA) were crosslinked on poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) for transporting etoposide across the blood-brain barrier (BBB) and treating human brain malignant glioblastoma. Lf- and FA-grafted PLGA NPs (Lf/FA/PLGA NPs) were employed to permeate the monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by human astrocytes an...

Biodegradable poly-(D,L-lactide-co-glycolide) microspheres (PLGA-MS) are approved as a drug delivery system in humans and represent a promising antigen delivery device for immunotherapy against cancer. Immune responses following PLGA-MS vaccination require cross-presentation of encapsulated antigen by professional antigen presenting cells (APCs). While the potential of PLGA-MS as vaccine formul...

Poly(D,L-lactide-co-glycolide) (PLGA) is the most frequently used biodegradable polymer for developing nano/ microparticles encapsulating therapeutic drugs in controlled release (CR) applications. PLGA based drug delivery devices have several advantages over the conventional devices. One of the advantage is the extended release rates of drugs up to days, weeks or months. Other reasons for the w...

Objective(s): In the current work, poly D, L lactide-co-glycolide (PLGA) particles were applied for a viral vaccine for the delivery of antigens in cytosolic pathway by increasing the antigen presentation to T-lymphocytes. HPV-E7 protein with PLGA particles has been reported as a potent adjuvant for HPV vaccine by encapsulating protein into the PLGA particles. Polysaccharide from Pleurotus sajo...

The aim of this work was to synthesize sulfadiazine-poly(lactide-co-glycolide) (SUL-PLGA) nanoparticles (NPs) for the efficient delivery of 5-fluorouracil to cancer cells. The SUL-PLGA conjugation was assessed using FTIR, 1H-NMR, 13C-NMR, elemental analysis and TG and DTA analysis. The SUL-PLGA NPs were characterized using transmission and scanning electron microscopy and dynamic light scatteri...

Recently, polymeric nanoparticles (NPs) have attracted enormous attention as targeted drug delivery vehicles.[1-4] Especially, biodegradable and biocompatible polymeric NPs comprised of poly(lactide-co-glycolide)-b-polyethyleneglycol (PLGA-PEG) block copolymers exhibit optimal physicochemical characteristics such as the ability to incorporate various targeting agents, enhanced immune evasion, c...

The objective of the study was to assess cytotoxicity (based on dimethylthiazol–diphenyltetrazolium bromide cell viability assay) and antimicrobial effects poly(lactictide-co-glycolide) nanoparticles with entrapped mupirocin (PLGA/MUP NPs) Staphylococcus aureus methicillin-resistant S. (MRSA) strains using a disk-diffusion method, cryo-scanning electron microscopy (cryo-SEM) fluorescence micros...

Using an electrospinning technique, we constructed composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA, ratio 85:15) and 33% wt.% of nanodiamond particles. The number of initially adhering human osteoblast-like MG 63 cells on day 1 after seeding, their spreading and subsequent growth were similar on both types of membranes. However, higher cell numbers on day...

Poly(lactide-co-glycolide) (PLGA) nanofibrous composite scaffolds having nano-hydroxyapatite particles (HAp) in the fibers were prepared by electrospinning of PLGA and HAp with an average diameter of 266.6 ± 7.3 nm. Microscopy and spectroscopy characterizations confirmed integration of the crystalline HAp in the scaffolds. Agglomerates gradually appeared and increased on the fiber surface along...

BACKGROUND To examine the effects of the addition of autologous platelet-rich plasma (PRP) into bilayer poly(lactide-co-glycolide) (PLGA) scaffolds on the reconstruction of osteochondral defects in a rabbit model. MATERIAL AND METHODS Porous PLGA scaffolds were prepared in a bilayered manner to reflect the structure of chondral and subchondral bone. Bone defects, measuring 4 mm in diameter and ...