Salivary gland cells are joined by junctional complexes consisting of a tight junction (TJ), zonula adherens and one or more desmosomes. TJs regulate paracellular permeability, maintain separate apical and basolateral membrane domains, and serve as signaling centers. We examined TJs of mouse submandibular glands (SMG) in thin sections and freeze-fracture replicas. TJs between acinar cells and b...

BACKGROUND The aim of the study is to detect apoptosis in granular cell ameloblastoma by annexin v affinity assay, a novel technique. MATERIALS & METHODS Excitional biopsy of two patients with granular cell ameloblastoma were fixed in buffered formalin and later embedded in paraffin wax. Blocks were sliced into 3m thick sections for routine histological and subsequent immunohistochemical exam...

A neoplastic nodular lesion consisting of an admixture of granular cell tumor and adenocarcinoma was found in the uterus of a 26-month-old Djungarian hamster. Neoplastic cells of the uterine adenocarcinoma showed an epithelial nature in their growth patterns and by cytokeratin-immunopositive reaction, exhibiting nuclear pleomorphism. The granular cells had an abundant amount of fine granular eo...

Animal studies have shown that prenatal stress is able to induce long-lasting neurobiological and behavioral alterations in adult offspring. In spite of the facts that hippocampus is sensitive to early developmental influences and its known functional importance in learning and memory, few data are available on the effect of prenatal stress on the structure of hippocampus. Therefore, this study...

Livers from human fetuses between the 16th and 24th weeks of gestation were dissociated by successive dispase and collagenase digestion followed by two cycles of low-speed differential centrifugation. This improved method recovered approximately 1 X 10(7) cells (90% hepatocytes and 90% viable cells) from 4 g of liver tissue. These hepatocytes were set into primary culture and monolayer granular...

Granular cell tumors are relatively uncommon benign lesions occurring in almost any part of the body. We report the cytological diagnosis of granular cell tumor in 25-year-old male patient who presented with an inguinal mass clinically suspected to be a lymphadenopathy. Fine needle aspiration revealed polygonal cells with abundant, granular cytoplasm and eccentrically located vesicular nuclei a...

Human peripheral blood lymphocytes that express T cell markers, when reacted for the cytochemical localization of lysosomal acid hydrolases, display two major patterns of staining, i.e., dot-like and scattered granular. Previous attempts to fractionate T cells according to surface markers have yielded populations of cells with heterogeneous patterns of cytochemical staining. In this study, peri...

Postmortem examination of a 5.5-year-old Holstein cow with history of sudden death showed a veryhuge spleen (120 × 45 × 10 cm) associated with severe haemoperitoneum. Histopathological findingsincluded extensive infiltration of medium to large sized lymphocytic and lymphoblastic cells with smallamounts of cytoplasm, round nuclei with coarsely granular chromatin in the spleen, and limited foci o...

The generation cycle of germinative cells (external matrix cells) in the external granular layer of the cerebellar cortex of the 10-to 11-day-old mouse was studied by radioautography following repeated injections of H(3)-thymidine. The generation time is 19 hr, presynthetic time 8.5 hr, DNA-synthetic time 8 hr, postsynthetic time 2 hr, and mitotic time 0.5 hr. These proliferating cells occupy t...