A novel strategy was proposed for Ramos cell detection by combining the expression of enhanced green fluorescent protein (eGFP) with the cell aptamer recognition and the triplex molecular beacons. This system was successfully applied to cancer cell detection with high sensitivity and specificity.

Dimerization-dependent fluorescent proteins (ddFP) are a recently introduced class of genetically encoded reporters that can be used for the detection of protein interactions in live cells. The progenitor of this class of tools was a red fluorescent ddFP (ddRFP) derived from a homodimeric variant of Discosoma red fluorescent protein. Here, we describe the engineering and application of an expan...

Fluorescent pigments in several Indo-Pacific and Caribbean anthozoans have recently been identified as proteins related to the green-fluorescent protein (GFP) of the hydromedusa Aequorea victoria. Here we show that GFP is widely distributed in many Caribbean species. The fluorescence excitation and emission spectra for the pigment are similar to those reported elsewhere for coral and noncoral G...

The light-harvesting complexes (LHCs) of land plants and green algae have essential roles in light capture and photoprotection. Though the functional diversity of the individual LHC proteins are well described in many land plants, the extent of this family in the majority of green algal groups is unknown. To examine the evolution of the chlorophyll a/b antennae system and to infer its ancestral...

ECERIFERUM1 (CER1) and ECERIFERUM3 (CER3) are key genes in synthesis of alkanes, a major component cuticular waxes land plants. The share extensive similarity, including the N-terminal (ERG3/FAH) C-terminal (WAX2) domains. This study, traces origin, evolutionary history, phylogenetic relationships variation copy number two within beyond Viridiplantae (green plants). Protein homologs both CER1 C...

The extracellular matrix of scaly green flagellates consists of small organic scales consisting of polysaccharides and scale-associated proteins (SAPs). Molecular phylogenies have shown that these organisms represent the ancestral stock of flagellates from which all green plants (Viridiplantae) evolved. The molecular characterization of four different SAPs is presented. Three SAPs are type-2 me...