Plasma membranes isolated from bovine corpora lutea showed specific binding with 1251-human chorionic gonadotropin and 1251-human luteinizing hormone; unlabeled hormones competitively inhibited the binding. The binding of the 1251-human chorionic gonadotropin to the receptor was a saturable phenomenon and the half-saturation was attained at a concentration of 4 X lo-r0 M. Maximum hormone recept...

An adrenocorticotropic hormone (ACTH)-secreting mouse pituitary tumor cell line was used to investigate the structure of the smallest glycoprotein form of ACTH. AtT-20/D16v cells were incubated with a “H-amino acid or “H-sugar; “H-labeled ACTH was prepared by double-antibody immunoprecipitation of culture medium with affinity purified (~(1-24) ACTH antiserum. Immunoprecipitates were solubilized...

Plasma membranes isolated from bovine corpora lutea showed specific binding with 1251-human chorionic gonadotropin and 1251-human luteinizing hormone; unlabeled hormones competitively inhibited the binding. The binding of the 1251-human chorionic gonadotropin to the receptor was a saturable phenomenon and the half-saturation was attained at a concentration of 4 X lo-r0 M. Maximum hormone recept...

A minimalist Go-model, with no energetic frustration in the native conformation, has been shown to describe accurately the folding pathway of the beta-trefoil protein, interleukin-1beta (IL-1beta). While it appears that these models successfully model transition states and intermediates between the unfolded and native ensembles, it is unclear how accurately they capture smaller, yet biologicall...

A 6 M guanidine-HCl/0.2 M EDTA solution was used to lyse and store whole blood specimens. DNA stored in guanidine-EDTA-blood (GEB) lysate was found to be undegraded after incubation at 37 degrees C for 1 month, suggesting that this represents an appropriate reagent for transport of blood samples from the field to a laboratory for analysis. Trypanosoma cruzi kinetoplast DNA in GEB lysate can be ...

The molecular weight of IgG3 myeloma proteins is about 170,000 as judged by gel filtration analyses of the light and heavy chains in 5 M guanidine-1 M acetic acid. The heavy chain of IgG3 has a molecular weight of about 60,000, which is larger by 9,000 to 10,000 than the molecular weight of the heavy chain of IgGl. Under nondenaturing conditions, an apparent molecular weight of 200,000 is obtai...

Treatment of adrenal iron-sulfur protein (adrenodoxin) with urea and guanidine HCI results in a deterioration reaction that proceeds through at least two intermediate stages yielding the completely denaturated, colorless protein. The first transient state exhibits a circular dichroism maximum at 470 rnp which is shifted to a longer wave length than that of the native protein (440 mp) ; at 470 r...

The primary structure of a 22-kDa protein which was isolated during the purification of bovine skin dermatan sulfate proteoglycan is described. The uronate-rich fraction from DEAE-Sepharose chromatography of a 7.8 M urea extract of bovine fetal skin was subjected to gel filtration on Sepharose CL-6B in 4 M guanidine HCl. A prominent component of mass 22 kDa was separated from the proteoglycan a...