To obtain stable expression of a foreign gene, it is important to have optimized conditions for plant genetic transformation. Here, a GUS intron-containing gene driven by CaMV 35S promoter was used to optimize the conditions of biolistic transformation of banana immature embryos and also for the study of GUS localization in the transformed cells. The GUS activity detected histochemically and fl...

Six transgenic tobacco lines, each homozygous for the beta-glucuronidase (GUS) gene at a different locus, and wild type were selfed and intercrossed to evaluate GUS activity in all possible hemizygous, homozygous and dihybrid combinations of GUS alleles. The transgenic lines are characterized by their GUS activity (two low, three intermediate, one high), T-DNA complexity (four single-copy, two ...

Transformed Arabidopsis plants were used to study the effect of the cauliflower mosaic virus (CaMV) inclusion body protein on translation of dicistronic RNA. Reporter plants contain a dicistronic transcription unit with CaMV open reading frame VII (ORF VII) as the first and the [beta]-glucuronidase (GUS) reporter ORF as the second cistron. "Transactivator plants" contain CaMV ORF VI under the c...

Drought is one of the most significant abiotic stresses that influence plant growth and development. Expression analysis revealed that OsNRT1.3, a putative nitrate transporter gene in rice, was induced by drought. To confirm if the OsNRT1.3 promoter can respond to drought stress, a 2019 bp upstream sequence of OsNRT1.3 was cloned. Three OsNRT1.3 promoter fragments were generated by 5'-deletion,...

Mutant alleles of two genetic regulatory elements, which underlie a three- to sixfold reduction in beta-glucuronidase (GUS) activity levels, distinguish mice of the H haplotype from those of the other two common GUS haplotypes, A and B. Both elements are tightly linked to the GUS structural gene over which they exert control. One (Gus-u) exerts a cis-active effect upon GUS activity levels in al...

The GUS gene of E. coli, encoding b-glucuronidase, has been widely used as a reporter gene in plant transformation. However, b-glucuronidase activity in transgenic wheat leaf or root tissue is rarely observed or reported. To address this question, we investigated three wheat lines transformed with the GUS reporter gene. We found all three lines expressed GUS mRNA as well as b-glucuronidase prot...

The 5' region of the rice actin 1 gene (Act l ) has been developed as an efficient regulator of foreign gene expression in transgenic rice plants. To determine the pattern and level of rice Actl 5' region activity, transgenic rice plants containing the Actl 5' region fused to a bacterial 8-glucuronidase (Gus) coding sequence were generated. Two independent clonal lines of transgenic rice plants...

A tropical endophytic isolate of the fungus Fusarium verticillioides (synonym Fusarium moniliforme) obtained from Zea mays was co-transformed with plasmid pNH24 containing the Fusarium oxysporum nitrate reductase nia gene and plasmid pNOM 102 carrying the Escherichia coli β-glucuronidase gusA gene. Transformation frequency for the nia marker was 75 transformants μg vector DNA and introduction o...

Major Accomplishments to date: 1. Genetic Manipulations. The fungal transformation expression vector, pBARGPE1 was provided by Dr. Krishna Podila, Michigan Technological University. The vector had the Aspergillus nidulans gpdA promoter and the A. nidulans trpC terminator flanking a multiple cloning site. The ß-glucuronidase gene (GUS) was provided by Dr. Randy Allen, Texas Tech University. The ...

We constructed a chimeric gene consisting of a soybean small auxin up RNA (SAUR) promoter and leader sequence fused to an Escherichia coli [beta]-glucuronidase (GUS) open reading frame and a 3[prime] untranslated nopaline synthase sequence from Agrobacterium tumefaciens. This chimeric gene was used to transform tobacco by Agrobacterium-mediated transformation. In R2 etiolated transgenic tobacco...