PURPOSE To determine whether, by employing recent advances in immunocytochemical technique, it is possible to identify reliably the product of the retinoblastoma (RB) susceptibility gene, p110RB1, in formalin-fixed, paraffin-embedded eyes with commercially available primary antibodies. If so, the authors sought to determine the distribution of p110RB1 in normal human eyes and retinoblastomas in...

BACKGROUND Reactive oxygen species (ROS) have been implicated in the turnover of epithelial cells in the rat intestine. The metabolism of ethanol generates ROS, which are implicated in cellular injury, but the levels of lipid peroxidation in intestine in chronic alcoholism are unknown. AIM To investigate the effects of ethanol ingestion on lipid peroxidation, and anti- and pro-oxidant enzyme ...

This work had as its objective the production of polyoma virus (PV) DNA of high specific radioactivity. Such a highly labeled tumorogenic virus DNA would have many applications in physical, chemical, and biological studies where high sensitivity of detection is essential-for example, for studies on the properties and fate of the viral gene after infection of a cell. This material has been produ...

The formation of a soluble H(3)-thymidine derivative pool has been examined in Tetrahymena pyriformis as a function of macronuclear DNA synthesis during the cell life cycle. An autoradiographic technique which allows the detection of water-soluble materials within a cell has shown that these cells do not take up and retain exogenous H(3)-thymidine during G1 or G2. Uptake of H(3)-thymidine is re...

A considerable decrease (24 to 40%) of DNA content per nucleus previously observed in the adrenal medulla of rats exposed intermittently to cold is followed by restoration to normal and supranormal values. This phenomenon has now been studied by use of H(3)-thymidine, which was given to normal rats, to rats exposed to cold, and to animals brought to room temperature after cold exposure. In the ...

Cell proliferation in the pineal body of the hooded rat was studied with thymidine-H:' autoradiography. Animals were either injected at birth and allowed to survive for variable periods or were injected at different ages and allowed to survive for a fixed period. Cell proliferation was high in the neonate (one and six hours of age) and continued at a decreasing rate into adulthood. The final de...

HE very specialized meiotic events that occur in all sexually reproducing organisms have not been investigated in any great detail by means of high resolution tritium autoradiography. Most of the work so far has been done with animals because of technical difficulties in obtaining uptake of labelled nucleosides in the microsporocytes of plants (TAKATS 1962). LIMA-DE-FARIA (1959, 1961,1962) has ...

This study was designed to answer the question: Is H3-thymidine uptake by nuclei of the mouse seminal vesicle evidence for DNA synthesis and mitosis, or does it signify some "metabolic" function of DNA unrelated to chromosome duplication? Mice were given an intraperitoneal injection of H3-thymidine. Six hours later Feulgen squashes of the seminal vesicle epithelium were made and covered with au...