The interaction between normal or glycated haemoglobin and the cytoplasmic surface of human erythrocyte ghost membranes from normal and diabetic individuals was studied at low pH and low ionic strength. Haemoglobin binding to the membrane was monitored by quenching of a fluorescent probe, 12-(9anthroyl) stearic acid, embedded in the membrane. The quenching occurs by energy transfer from the pro...

Agar gel, cellulose acetate, and starch gel electrophoresis are all capable of diagnosing sickle-cell anaemia, sickle-cell haemoglobin C disease, and haemoglobin C disease in cord blood samples. Of these three electrophoretic techniques, agar gel is the easiest to interpret.Paper electrophoresis can reliably and rapidly detect sickle haemoglobin and haemoglobin C in cord blood samples. Being in...

Two interrelated aspects of haemoglobin differentiation in vertebrates are tissue specificity (muscle haemoglobin and blood haemoglobin) and time specificity (embryonic, larval or foetal haemoglobin, and adult haemoglobin). Tissue specificity of haemoglobin and of other respiratory proteins has been found in invertebrates and has been shown to be the result of differences in gene activity (Man ...

Aims: To assess the accuracy and precision of measuring haemoglobin A2 by high performance liquid chromatography (HPLC) in the presence and absence of sickle cell trait, with or without a thalassaemia trait. Methods: The haemoglobin A2 percentage and the haemoglobin A2 plus S percentages were determined by HPLC on 82 normal controls and 78 patients with sickle cell trait, respectively; the a th...