ten female lambs of 7-month-old were divided into two equal groups and raised under a helminth-free conditions. animals in group 1 were immunized two times by whole gut homogenate (wgh) of haemonchus contortus emulsified in freund’s adjuvant. in group 2 (control), animals were injected by phosphate buffered saline emulsified in the same adjuvant. animals were challenged by 10000 third-stage lar...

Lambs vaccinated with Haemonchus contortus excretory/secretory (ES) glycoproteins in combination with the adjuvant Alhydrogel are protected against H. contortus challenge infection. Using glycan micro-array analysis we showed that serum from such vaccinated lambs contains IgG antibodies that recognise the glycan antigen Galalpha1-3GalNAc-R and GalNAcbeta1-4(Fucalpha1-3)GlcNAc-R. Our studies rev...

Genetic variability of the ovine parasite Haemonchus contortus from the Alpine area was investigated using mitochondrial DNA (nd4 gene), internal transcribed spacers 1 and 2 and microsatellites, in order to assess whether cross-transmission between domestic and wild ruminants occurs. The dataset was composed of 78 individual adult male H. contortus collected from chamois (Rupicapra r. rupicapra...

The seasonal incidence of helminth infestation on a farm in the Transvaal Highveld was determined by the slaughter of tracer lambs exposed on pasture for periods of approximately 6 weeks. Haemonchus contortus, the most prevalent nematode, was recovered in the greatest numbers from January--May 1974. Peak counts of Ostertagia circumcincta were made during March and April 1974 and in January 1975...

A cambendazole-resistant (CR) strain of Haemonchus contortus, the large stomach nematode of sheep, had similar in vitro growth characteristics compared to the parent strain (BPL). When the in vitro cultivation system was modified to detect drug resistance: (1) exsheathed infective larvae of the BPL strain did not develop in a culture medium with a concentration of 2.5 /ug/rnl thiabendazole (TBZ...

The known chromodorolides A (1) and B (2), and a new derivative chromodorolide C (3) have been isolated from an Australian sponge and characterized by 1D and 2D NMR experiments. The chromodorolides exhibited significant cytotoxicity against the P388 mouse leukaemia cell line, and also showed activity against the freeliving larval stages of the parasitic nematodes Haemonchus contortus and Tricho...

BACKGROUND This study investigated seasonal changes in naturally acquired gastrointestinal nematode (GIN) infections on two Lithuanian goat farms with different parasite control practices. FINDINGS On both farms, nematode faecal egg counts (FEC) and larval cultures were obtained from 15 adult and 10 young goats at bi-weekly intervals from April 2012 to April 2013. Goats on farm A were deworme...

Resistance to eprinomectin was suspected in a dairy sheep farm southwestern France. The efficacy of topical and injection formulations against gastrointestinal nematodes (GINs) compared using faecal egg count reduction test. GIN species were identified by real-time PCR, concentrations measured serum High-Performance Liquid Chromatography 2 5 days after treatment. Efficacies 99.6% 86.1% for form...

Diagnosis is often equated with identification or detection when discussing parasitic diseases. Unfortunately, these are not necessarily mutually exclusive activities; diseases and infections are generally diagnosed and organisms are identified. Diagnosis is commonly predicated upon some clinical signs; in an effort to determine the causative agent, identification of genera and species is subse...

Two doses of infective larvae of 20 000 Trichostrongylus axei, dosed to Merino lambs at an interval of 14 days and subsequently challenged with Ostertagia circumcincta, caused a significant reduction (P less than 0.01) in the establishment of 5th and adult stages of the latter. T. axei was unable to protect Merino sheep against homologous challenge nor was Haemonchus contortus a successful vacc...